人脑星形细胞肿瘤IL-8的表达及其与血管生成的关系

目的：探讨IL-8,VEGF在脑胶质瘤中的表达及与血管生成的关系。方法：应用由45例人脑星形细胞肿瘤和6例正常脑组织组成的组织芯片，采用免疫组织化学技术分别进行IL-8，VEGF，CD34标记并进行半定量，观测在不同病理分级胶质瘤中的表达及与微血管密度（MVD）之间的关系。结果：6例正常脑组织中不表达IL-8，VEGF。45例脑胶质瘤中，27例IL-8呈阳性表达，32例VEGF呈阳性表达，Ⅲ，Ⅳ级脑胶质瘤中IL-8，VEGF的表达比Ⅰ级、Ⅱ级明显增强，IL-8，VEGF评分为强阳性的胶质瘤内MVD明显高于评分为阴性和阳性的MVD（P<0.01）。IL-8表达与MVD呈正相关（rs=0.64，P<0.01）。VEGF表达与MVD之间呈正相关（rs=0.44，P<0.01）。IL-8表达与VEGF表达之间亦呈正相关（rs=0.56，P<0.01）。结论：IL-8，VEGF的表达与胶质瘤病理分级、MVD密切相关，二者在胶质瘤的血管生成中可能相互关联、共同调节肿瘤血管生成。

Expression of IL-8 in astrocytomas and relationship with angiogenesis

Objective To explore the relationship between interleukin-8(IL-8) and vascular endothelial growth factor(VEGF) expression and angiogenesis in human gliomas.Methods Immunohistochemical staining was performed on the tissue array constructed with 45 cases of human astrocytomas and 6 cases of normal brains to detect expressions of IL-8,VEGF and CD34,and observe the correlation between IL-8 and VEGF expression levels and microvessel density(MVD) in astrocytomas with different pathologic grades.Results IL-8,VEGF in 6 cases of normal brain were undetectable.In 45 cases of gliomas,27 cases were IL-8 positive,32 cases VEGF positive. The MVD were significantly higher in IL-8 and VEGF positive cases than in mild positive or negative cases.The IL-8 and VEGF expressions were correlated with MVD.IL-8 expression was positively correlated with VEGF expression.Conclusion The expressions of IL-8 and VEGF have a correlation with histologic grade and MVD of astrocytoma,and both regulate the tumor angiogenesis in gliomas.