| 大孔树脂分离纯化荷叶中的黄酮与生物碱 |
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| 引用本文： | 刘东亚,王谊,蒋建勤. 大孔树脂分离纯化荷叶中的黄酮与生物碱[J]. 西北药学杂志, 2012, 27(6): 511-514 |
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| 作者姓名： | 刘东亚 王谊 蒋建勤 |
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| 作者单位： | 刘东亚 (中国药科大学天然药物化学研究室,南京,211198) ； 王谊 (中国药科大学天然药物化学研究室,南京,211198) ； 蒋建勤 (中国药科大学天然药物化学研究室,南京,211198) ； |
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| 基金项目： | 江苏省普通高校研究生科研创新计划项目 |
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| 摘 要： | 目的研究确定荷叶的最佳分离纯化工艺。方法采用不同的树脂柱进行吸附,用分光光度法测定黄酮与生物碱的含量,用HPLC验证荷叶碱的含量,对工艺进行评价。结果 D-101型树脂较适合荷叶中的生物碱与黄酮的分离与纯化,在确定的工艺下,荷叶碱转移得率达到57.2%。结论该工艺适合荷叶中的黄酮与生物碱的分离与富集。
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| 关 键 词： | 荷叶 黄酮 生物碱 大孔树脂 |
Isolation and purification of flavonoids and alkaloids from the leaf of Nelumbo nucifera Gaertn by macroporous adsorption resin |
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| LIU Dongya,WANG Yi,JIANG Jianqin. Isolation and purification of flavonoids and alkaloids from the leaf of Nelumbo nucifera Gaertn by macroporous adsorption resin[J]. Northwest Pharmaceutical Journal, 2012, 27(6): 511-514 |
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| Authors: | LIU Dongya WANG Yi JIANG Jianqin |
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| Affiliation: | (Department of Natural Medicinal Chemistry,China Pharmaceutical University,Nanjing 211198,China) |
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| Abstract: | Objective To investigate the purification of flavonoids and alkaloids from the leaf of Nelumbo nucifera Gaertn by macroporous adsorption resin.Method Different types of macroporous adsorption resin were used for adsorpting.Spectrophotometry was used to measure the total flavonoids and alkaloids,and HPLC was used to determine the content of nuciferine.Result The D-101 macroporous adsorption resin was suitable for the purification of flavonoids and alkaloids from the leaf of Nelumbo nucifera Gaertn.Under the optimized conditions,the recovery rate of nuciferine was 57.2%.Conclusion This method was suitable for the purification of flavonoids and alkaloids from the leaf of Nelumbo nucifera Gaertn. |
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| Keywords: | leaf of Nelumbo nucifera Gaertn flavonoids alkaloids macroporous adsorption resin |
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