白介素12A基因多态性与OLP的相关性研究

目的：研究华东地区汉族人群IL-12A基因多态性与口腔扁平苔藓（orallichenplanus，OLP）的相关性。方法：采用TaqMan荧光定量PCR法检测华东地区292例OLP患者及686例正常对照者的IL-12A基因的5个SNP位点（rs3024415，rs2243123，rs583911，rs568408和rs2243143），分析IL-12A基因多态性与OLP的相关性。结果：①华东地区健康人群IL-12A基因3′-UTRrs568408位点AA、GA和GG基因型频率分别是0．7％、12．4％和86．8％，而OLP患者分别是1．1％、18．4％和80．5％，其中GA和AA基因型分布频率显著高于正常对照组@=O．0427）。②正常对照组IL-12A基因rs583911位点AA、GA和GG基因型频率分别是4．2％、38．6％和57．1％，而OLP患者分别是9．5％、36．5％和54％，其中AA基因型分布频率显著高于对照组（p=O．00805）。③糜烂型OLP组IL-12A—rs568408／A等位基因频率显著高于正常对照组，分别为11．6％和6．94％（OR=I．76，95％CI：1．133—2，732，P=0．011）。结论：华东地区汉族OLP人群IL-12A基因rs568408位点存在多态性变异，可能与OLP的疾病易感性和严重程度有关。

Association study of interleukin 12A gene polymorphisms with oral lichen planus

Objective：To investigate single nucleotide polymorphisms （SNP） of interleukin 12A gene with oral lichen planus （OLP） in Chinese Hans residing in East and South-east China, and to provide an important genetic basis in exploring the disease susceptibility and diverse clinical manifestation. Method：Two hundred and ninety-two patients with OLP and 686 healthy control subjects were recruited in Chinese Hans residing in East and South-east China. Genomic DNA was ex- tracted and PCR products spanning five SNPs of the IL-12A gene at positions （rs3024415, rs2243123, rs583911, rs568408 and rs2243143） were analyzed. The genotype frequencies were calculated and analyzed. Result：①Polymorphism of rs568408, located at the 3 ′-UTR region was found to have a significant association with OLP （P =0.0427）. ②Polymorphism of rs583911 was also found to have a significant association with OLP （P =0.0085）. ③The allele frequencies of IL-12A- rs568408 / A in the erosive O LP group were much higher than that in the healthy group （OR= 1.76,95 % CI： 1.133-2.732, P =0.011）. Conclusion： The variation of IL-12A rs568408 gene polymorphisms seems to have some influence on the disease susceptibility and association with the severity of OLP in this Chinese cohort residing in East and South-east China.