| 四色流式分析多发性骨髓瘤细胞免疫表型及稳定性 |
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| 引用本文： | 苏倩倩,谢晓宝,邱国强.四色流式分析多发性骨髓瘤细胞免疫表型及稳定性[J].临床血液学杂志,2011,24(3). |
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| 作者姓名： | 苏倩倩 谢晓宝 邱国强 |
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| 作者单位： | 苏倩倩 (苏州大学附属第三医院血液科,江苏常州,213003) ； 谢晓宝 (苏州大学附属第三医院血液科,江苏常州,213003) ； 邱国强 (苏州大学附属第三医院血液科,江苏常州,213003) ； |
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| 摘 要： | 目的:探讨多发性骨髓瘤(MM)细胞的免疫表型特征及稳定性。方法:用四色流式细胞术检测28例MM患者恶性浆细胞表面免疫表型,并对其中12例MM初诊患者在2～4m非单抗治疗后重复检测免疫表型。结果:与正常浆细胞不同,多数MM恶性浆细胞异常表达CD56(89.3%)、CD117(50.0%)、CD28(25%)、CD20(14.3%),不表达CD19(96.4%),近一半的患者不表达CD27(42.8%);恶性浆细胞表面免疫表型不稳定,12例初诊患者中有5例(41.67%)患者化疗后抗原表达有改变;CD38++CD45dim～-CD19-CD56+表型的恶性浆细胞所占圈定浆细胞百分率(NP)与骨髓形态学检测瘤细胞负荷有很强的正相关性(γ=0.675,P<0.01),并且可以区分稳定期和进展期患者(P<0.05)。结论:恶性浆细胞表面异常表达CD56,不表达CD19,部分表达CD117、CD28、CD20,近一半患者CD27表达缺失。部分初诊患者非单抗治疗后,恶性浆细胞表面抗原表达发生变化。NP可以稳定区分稳定期和进展期患者,是衡量病情的新指标。
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| 关 键 词： | 多发性骨髓瘤 流式细胞术 免疫表型 稳定性 |
Four-color flow cytometric analysis of myeloma plasma cells and their stability |
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| SU Qianqian XIE Xiaobao QIU Guoqiang.Four-color flow cytometric analysis of myeloma plasma cells and their stability[J].Journal of Clinical Hematology,2011,24(3). |
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| Authors: | SU Qianqian XIE Xiaobao QIU Guoqiang |
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| Institution: | SU Qianqian XIE Xiaobao QIU Guoqiang(Department of Hematology,the Third Hospital of Suzhou University,Changzhou,213003,China) |
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| Abstract: | Objective:This study was purposed to investigate the immunophenotype characteristics in multiple myeloma(MM) cells and their stability.Method:BM aspirate samples from 28 newly diagnosed MM patients and 12 patients of them after treatment were assessed using 4 color flow cytometric analyses.The plasma cells(PCs) were identified by their characteristic light scatter distribution and reactivity patterns to CD138,CD38,and CD45.Result:Moderate to bright expression of CD56,CD117,CD28,and CD20 was detected in 89.3... |
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| Keywords: | multiple myeloma flow cytometry immunophenotype stability |
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