超滤-反渗透集成工艺浓缩黄芩水提液的可行性

目的:探讨超滤-反渗透浓缩黄芩水提液的可行性。方法:黄芩水提液经离心、超滤及反渗透处理,通过HPLC特征图谱考察处理前后黄芩水提液中主要化学组成的变化,流动相甲醇(A)-0.05%磷酸水溶液(B)梯度洗脱(0~35 min,40%A;35~75 min,60%A),检测波长280 nm。考察反渗透膜的清洗液(碱液、酸液、十二烷基苯磺酸钠溶液及纯水)。结果:浓缩4 h后,经预处理的药液浓缩倍数为3.2倍,通量衰减了67.64%;未经预处理的药液浓缩倍数为1.6倍,通量衰减了85.35%。浓缩后药液中黄芩苷质量浓度由1.03 g·L-1增至8.87 g·L-1。各试液相似度均0.99,说明浓缩前后样品的相似性良好。采用不同清洗液清洗时,反渗透膜的通量恢复率依次为87.73%,81.50%,67.65%,93.35%,选择纯水清洗反渗透膜。结论:超滤-反渗透集成工艺化学成分保留率、浓缩效率高,适用于黄芩水提液的有效浓缩。

Feasibility Analysis for Integrated Technology of Ultrafiltration and Reverse Osmosis for Concentration of Scutellariae Radix Decoctions

Objective: To investigate feasibility of concentration of Scutellariae Radix decoctions by integrated technology of ultrafiltration and reverse osmosis. Method: Scutellariae Radix decoctions was treated in turn by centrifuging,ultrafiltration,reverse osmosis.HPLC was adopted to compare main chemical composition of samples before and after treatment,mobile phase was methanol(A)-0.05% phosphoric acid(B) for gradient elution(0-35 min,40%A;35-75 min,60%A),detection wavelength was set at 280 nm.In addition,NaOH solution,HCl solution,sodium dodecyl benzene sulfonate solution and pure water are respectively used to clean membrane fouling. Result: After concentrated 4 hours,concentration ratio of pretreated decoctions was 3.2 times and flux attenuation was 67.64%;concentration ratio of decoctions without pretreatment was 1.6 times and flux attenuation was 85.35%.Contents of baicalin before and after being concentrated were 1.03 g· L^-1 and 8.87 g· L^-1,respectively.Similarities of all test solutions were more than 0.99,it showed that samples before and after concentration had good similarity.After cleaned by different cleaning fluids,flux recovery rates of reverse osmosis were 87.73%,81.50%,67.65%,93.35%,so taking pure water to clean membrane fouling. Conclusion: Integrated technology of ultrafiltration and reverse osmosis has advantages of high retention rate of chemical ingredients and high concentration efficiency,which can be used to concentrate Scutellariae Radix decoctions.