| Activation of NF-κB in bronchial epithelial cells from children with asthma |
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| 摘 要： | Objectives To determine whether nuclear factor-κB (NF-κB) is activated in epithelial cells from children with asthma and to understand the role of NF-κB in airway inflammation in asthma. Methods Bronchial mucosa specimens were obtained from 9 children with asthma and 6 control subjects. NF-κB expression in epithelial cells were detected by immunohistochemical examination, and NF-κB-DNA binding was measured by electrophoretic mobility shift assay (EMSA). Results Nuclear expression of NF-κB in epithelial cells was observed in the 9 asthmatic children. NF-κB-DNA binding was found in 4 asthmatic children (EMSA was performed in 6 asthmatic children). In contrast, both nuclear expression and NF-κB-DNA binding were absent in the 6 control subjects. Conclusion These results indicated that NF-κB is activated in epithelial cells from asthmatic children and the NF-κB activation may be the basis for the increased expression of many inflammatory genes and for airway inflammation in asthma.
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| 关 键 词： | nuclear factor-κB epithelial cells asthma |
Activation of NF-kappa B in bronchial epithelial cells from children with asthma. |
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| Authors: | ZHAO Shunying QI Yu LIU Xicheng JIANG Qinbo LIU Shiying JIANG Ying JIANG Zaifang |
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| Institution: | Pulmonary Function Laboratory, Beijing Institute of Pediatric Research, Beijing 100045, China;Central Laboratory, First Clinical Hospital, Peking University, Beijing 100034, China;Pulmonary Function Laboratory, Beijing Institute of Pediatric Research, Beijing 100045, China;Pulmonary Function Laboratory, Beijing Institute of Pediatric Research, Beijing 100045, China;Pulmonary Function Laboratory, Beijing Institute of Pediatric Research, Beijing 100045, China;Pulmonary Function Laboratory, Beijing Institute of Pediatric Research, Beijing 100045, China;Pulmonary Function Laboratory, Beijing Institute of Pediatric Research, Beijing 100045, China |
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| Abstract: | OBJECTIVES: To determine whether nuclear factor-kappa B (NF-kappa B) is activated in epithelial cells from children with asthma and to understand the role of NF-kappa B in airway inflammation in asthma. METHODS: Bronchial mucosa specimens were obtained from 9 children with asthma and 6 control subjects. NF-kappa B expression in epithelial cells were detected by immunohistochemical examination, and NF-kappa B-DNA binding was measured by electrophoretic mobility shift assay (EMSA). RESULTS: Nuclear expression of NF-kappa B in epithelial cells was observed in the 9 asthmatic children. NF-kappa B-DNA binding was found in 4 asthmatic children (EMSA was performed in 6 asthmatic children). In contrast, both nuclear expression and NF-kappa B-DNA binding were absent in the 6 control subjects. CONCLUSION: These results indicated that NF-kappa B is activated in epithelial cells from asthmatic children and the NF-kappa B activation may be the basis for the increased expression of many inflammatory genes and for airway inflammation in asthma. |
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