马鬃蛇对下丘脑抑制大鼠性功能的改善作用机制探讨

目的:研究马鬃蛇石油醚提取物(CVPEE)对下丘脑抑制的幼年雄性大鼠性功能的改善作用机制.方法:建立下丘脑抑制致大鼠睾丸功能障碍模型,连续灌胃给药21天后测定血清睾酮(T)、卵泡刺激素(FSH)水平,测定血清和阴茎组织中NO含量,换算其生殖器官的脏器系数,镜下观察睾丸、附睾的组织病理学改变.结果:CVPEE可明显升高血清睾酮水平,降低FSH水平,与模型组比较P＜0.05,血清和阴茎组织NO含量有升高趋势,给药组睾丸组织受损程度比模型组轻.结论:CVPEE可升高下丘脑抑制大鼠的血清睾酮水平及阴茎组织NO含量,对性功能障碍有一定改善作用,其作用机制可能通过下丘脑-垂体-性腺轴的调节.

Studies of Calotes versicolor on the Sexual Function Amelioration Mechanism by Hypothalamus Inhibition in Immature Male Rats

Objective: To study the amelioration mechanism of Calotes versicolor petroleum ether extracts(CVPEE) on hypothalamus inhibition sexual function induced by morphine in immature male rats.Methods: The hypothalamus inhibition induced by morphine in immature male rats was used as model of the testis reproductive functional disturbance. The rats was given CVPEE for 21 days.The levels of testosterone(T) and follicle stimulating hormone(FSH) in serum were determined by the radio-immunoassay.The nitrogen monoxide(NO) contents in serum and penis tissue were determined by nitrate reductase method.The sexual organ and auxiliary sexual organ coefficient were calculated and the histopathological changes in the testes and epididymides were observed with microscope.Results: CVPEE groups(low and high dosage groups) significantly enhanced the level of serum T, compared with model group(P<0.05); the low dosage group decreased obviously the level of FSH. CVPEE had the tendency to increase NO contents in serum and penile tissue.The microscopic examination results showed that the testis tissue in CVPEE group was damaged more gently than those in model group. Conclusion: CVPEE can enhance the level of serum T and NO contents in penile tissue, improve the sexual functional disturbance in hypothalamus inhibition induced by morphine in immature male rats.It indicated that CVPEE had the effects by the hypothalamus-hypophysis-gonad axial function.