Cloning and expression of MP13 gene from rat hippocampus, a new factor related to guanosine triphosphate regulation |
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Authors: | Feng Z Hong J Qi Q Han Y Wilson B Iadarola M Tiao N Bing G |
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Affiliation: | Division of Neurology, University Department of Medicine, University of Hong Kong, Queen Mary Hospital, Pokfulam Road, Hong Kong, People's Republic of China. |
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Abstract: | C-Fos and the Fos-related antigens (FRA) are induced by various stimuli. A novel 35-37 kDa FRA was induced much longer after the treatment using kainic acid (KA) and may be very important for neuronal survival after brain damage. To identify this long-term FRA, we have constructed a cDNA library derived from hippocampus after KA treatment and screened it with an antibody highly conserved M-peptide region of FRAs. One gene, MP13, was cloned with a 1662 bp open reading frame and coded for a 554-amino acid protein. MP13 has a leucine zipper region, a glutamine repeat region, and has high similarity to the activator of the small guanosine triphosphate (GTP)ase Rab5. Gel retardation analysis revealed that MP13 functions as a GTP regulation related factor. |
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