促红细胞生成素对脑出血神经生长因子表达的影响

目的研究促红细胞生成素(EPO)对脑出血后神经功能缺损、细胞凋亡及神经生长因子表达的影响。方法用立体定向技术,将自体不凝血注入大鼠尾状核区制备脑出血模型。110只Wistar雄性大鼠,随机分成四组:正常组、假手术组、ICH对照组、rhEPO治疗组。应用免疫组化及原位细胞凋亡检测脑出血灶周组织NGF、BDNF表达及凋亡细胞。统计学方法采用t检验和LSD-t检验。结果与对照组比较,rhEPO治疗后48～168 h大鼠神经行为学评分明显减少(P0.05)。ICH对照组及rhEPO治疗组术后6h血肿周围皮质TUNEL、NGF、BDNF阳性细胞明显增加,72h达到高峰,120h下降,各时间点与假手术组比较差异有显著性(P0.01)。rhEPO治疗组TUNEL阳性细胞数明显少于同时点ICH对照组(P0.01),但NGF、BDNF阳性细胞数明显增加(P0.01)。结论凋亡机制参与脑出血后继发性损伤过程,EPO能促进神经元BDNF、NGF的表达,对脑出血后神经损伤起保护作用。

The effect of erythropoietin on expression of nerve growth factor in intracerebral hemorrhage

【Objective】 We studied the effect of erythropoietin on neurological function deficit,neuron apoptosis and expression of nerve growth factor in rats after ICH.【Methods】 We produed model of ICH according to method of autoblood injecting caudate nucleus with stereo directionlism.110 male Wistar rats were randomLy divided into four groups,normal group,sham operation group,ICH group,EPO treatment group.The technichs of immunohistochemistry and TUNEL were appied to detect expression of NGF,BDNF and apoptosis cells.We appled t-test and LSD-t test to analysis data.【Results】 Contrast to ICH group,the score of neurological behavior in treatment group rats were decreased significantly at 48~168 hours（P0.05）.The positive cells of TUNEL NGF andBDNF in ICH group,EPO group obviously increased after 6h,reached peak 72h and decreased after 120h,the positive cells on different time points significantly decreased compared with that of in sham operation group（P0.01）.The positive cells of TUNEL in EPO group on different time points significantly decreased compared with that of in ICH group（P0.01）.The NGF and BDNF positive cells in EPO group on different time points significantly increased compared with that of in ICH group（P0.01）.【Conclusion】 Apoptosis mechanisms may mediate some of the brain injury after ICH,Erythropoietin can protect neurons from injury by increasing expression of BDNF and NGF.