探讨性别和年龄在慢性丙型肝炎患者外周血Toll样受体3和7基因表达中的影响

目的 观察不同性别和年龄慢性丙型肝炎(CHC)患者外周血单个核细胞(PBMC)中Toll样受体3(TLR3)和Toll样受体7(TLR7)mRNA表达,探讨性别和年龄在CHC患者外周血TLR3和TLR7基因表达中的影响。方法 选择CHC患者115例,健康人群113例,取抗凝全血,实时荧光定量PCR检测TLR3和TLR7 mRNA表达。结果 CHC患者TLR3和TLR7表达量与健康人群相比,差异均有统计学意义(t=38.73,6.16,P<0.05)。CHC患者中非绝经期女性TLR3和TLR7表达量与绝经期女性和青年男性相比,差异均有统计学意义(t=61.210,6.464,P<0.05; t=24.166,26.266,P<0.05)。CHC患者中老年男性TLR3和TLR7表达量与青年男性相比,差异均有统计学意义(t=86.349,19.583,P<0.05),而与绝经期女性相比,仅TLR3表达量差异有统计学意义(t=122.941,P<0.05)。不同性别和年龄CHC患者的TLR3,TLR7基因表达量与HCV-RNA载量均无相关性(|r|<0.40,P>0.05),但是青年女性CHC患者HCV含量均比青年男性和老年女性低,差异有统计学意义(t=3.49,2.51,P<0.05),老年男性患者HCV含量比青年男性低,差异有统计学意义(t=2.35,P<0.05),老年男性和女性CHC患者HCV-RNA含量差异无统计学意义(t=1.20,P>0.05)。结论 性别和年龄与CHC患者TLR的表达有一定的关系,为临床合理有效治疗CHC提供理论依据和新方法。

Exploring Impact of Gender and Age Discrepancies on the Expression of Toll-like Receptor 3 and 7 Gene mRNA of Patients with Chronic Hepatitis C Infection

Objective Investigating the expression of TLR3and TLR7 mRNA in peripheral blood mononuclear cells(PBMC)of patients with chronic hepatitis C infection(CHC),to explore the effects of gender and age on Toll-like receptor expression.Methods Peripheral blood wascollected from 115 patients of chronic hepatitis C infection,113 healthy individuals.Expression levels of TLR3 and TLR7 mRNA were detected by real-time quantitative PCR.Results The expression of TLR3 and TLR7 mRNA were significant difference between patients with CHC infection and healthy individuals(t=38.73,6.16,P<0.05),respectively.The expression of TLR3and TLR7 mRNA were significant difference between premenopausal females with CHC infection,postmenopausal females and young males of CHC infection(t=61.210,6.464,P<0.05; t=24.166,26.266,P<0.05),respectively.The expression of TLR3 and TLR7 mRNA were significant differences between old males andyoung males of chronic hepatitis CHC infection(t=86.349,19.583,P<0.05).The expression of TLR3 mRNA was significant differences between old males andpostmenopausal females of CHC infection(t=122.941,P<0.05).There was no correlation between the expressions of TLR3 and TLR7 and HCV-RNA load of CHCpatents of Gender and age discrepancies(|r|<0.40,P>0.05).The HCV-RNA load of premenopausal females was significant lower than young males and old females(t=3.49,2.51,P<0.05),the load of old males was lower thanold females(t=2.35,P<0.05),however,there was no significant differences between old males and old females(t=1.20,P<0.05).Conclusion Gender and age discrepancies have a relationship with the expression of Toll-like receptors of patients with CHC infection,which may provide atheoretical basis and a new method for CHC.