两种试剂D-二聚体检测值 与纤维蛋白降解产物值的相关性研究

目的 通过研究两种不同试剂D-二聚体(D-DIMER)检测值与纤维蛋白降解产物(fibrin degradation products,FDP)的相关性,分析检测值的吻合度,评价两种检测试剂的临床实用性。方法 采用Sysmex CA-1500全自动血凝分析仪检测患者D-二聚体及FDP,D-二聚体检测试剂分别为Siemens Innovance D-DIMER和Dade Behring D-Dimer Plus。回顾性研究762例临床患者标本D-二聚体及FDP测定结果,并将测定结果进行等级频数分析及等级相关检验(Spearman法)。结果Siemens Innovance D-DIMER试剂检测同一患者D-二聚体值与FDP水平呈正相关(r=0.954,P<0.01),优于Siemens DD Plus试剂(r=0.885,P<0.01),且这种相关性随D-二聚体含量的升高而升高,当D-二聚体>5 mg/L FEU时,相关性达到最佳(P<0.01)。Innovance D-DIMER试剂组FDP阳性率(约为65.76%)相对于DD Plus试剂组(91.48%)偏低,提示Innovance D-DIMER试剂组D-二聚体能够在FDP升高之前出现反应,具有更高的灵敏度。结论 使用Siemens Innovance D-DIMER试剂检测D-二聚体并结合FDP值可以为临床提供更加确切的疾病诊治信息。

Study of the Correlation of Testing Results by Two Different D-dimer Reagents with Fibrin Degradation Products Result Values

Objective To evaluate the clinical practicalityof two different D-dimer assays by studying the correlation of the testing results by two different D-dimer reagents with fibrin degradation products(FDP)result values and analyzing the Clinical alignment.Methods A representative cross-sectional study of 762 clinical patients' plasma specimens were taken to measure the results of D-dimer and FDP by using Sysmex CA-1500 automated coagulation analyzer,D-DIMER detection reagent respectively Siemens Innovance D-DIMER and Dade Behring D-DIMER Plus reagents.Then rating frequency analysis and Spearman correlation test were applied to analyze the correlation of measurement results.Results Overall,to the same patient,the correlation of D-dimer tested by Siemens Innovance D-DIMER Reagent with FDP level was significant positive(r= 0.954,P<0.01),better than that tested by Siemens DD Plus Reagent(r=0.885,P<0.01); and this correlation had an increasing trend when D-dimer level raised.When D-dimer level >5 mg/L FEU,the correlation was the best(P<0.01).And by calculating theamount of positive FDP(FDP>5 μg/ml)and FDP positive rate in both different cases when D-dimer results was also positive,FDP positive rate in Innovance D-DIMER Reagent group(about 65.76%)was lower than DD Plus Reagent group,it indicated that D-dimer results tested by Innovance D-DIMER Reagent could raise before FDP reacted,so D-dimer had a higher sensitivity than FDP.Conclusion It can provide a more precise clinical diagnosis and treatmentinformation when using Siemens Innovance D-DIMER Reagent to detect D-dimer values and combined FDP values at the same time.