| 细胞因子诱导不同来源的贴壁细胞分化为树突状细胞的研究 |
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| 引用本文： | 侯著法,陈梅芳,姚茂篪,梁辉.细胞因子诱导不同来源的贴壁细胞分化为树突状细胞的研究[J].湖北民族学院学报(医学版 ),2001,18(1):5-8. |
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| 作者姓名： | 侯著法 陈梅芳 姚茂篪 梁辉 |
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| 作者单位： | 1. 湖北民族学院医学院附院内科,
2. 上海第二医科大学附属新华医院造血干细胞移植科, |
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| 摘 要： | 目的：探讨寻树突状态细胞（DC）体外培养的理想来源。方法：通过贴壁的方法分离脐带血、造血动员后的外周血及正常人外周血中DC的前体细胞，体外采用细胞因子诱导，并对其进行形态学的观察和细胞表型检测；同时比较了不同来源的DC的增殖、分泌IL－12的能力及激发同种异体T细胞和脐带血幼雅T细胞（naive Tcells)的作用，结果：经过造血动员后的外周血产生的DC数量和纯度均较高，而且能经体外细胞因子诱导为功能性DC。结论：造血动员后的外周血是较理想的DC来源。
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| 关 键 词： | 树突状细胞 协同刺激因子 幼稚型T细胞 细胞因子 免疫应答 贴壁 |
| 文章编号： | 1008－8164(2001)01－0005－04 |
| 修稿时间： | 2000年12月25 |
The Study of Dendritic Cells Induced by Cytokines from Different Adherent Cells in Vitro |
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| HOU Zhu-fa,CHEN Mei-fang,YAO Mao-chi,et al.The Study of Dendritic Cells Induced by Cytokines from Different Adherent Cells in Vitro[J].Journal of Hubei Institute For Nationalities(Medical Edition),2001,18(1):5-8. |
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| Authors: | HOU Zhu-fa CHEN Mei-fang YAO Mao-chi |
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| Abstract: | Ojective To investigate the ideal sources of DC. cultured in vitro. Methods The adherent precursors of dendritic cells (DC) isolated from cord blood, mobilized or non-mobilized peripheral blood were induced with cytokines to DC in vitro. The growth of DC from different sources, and their potentials to stimulate the proliferation of na?ve t cells from cord blood were observed. Results It was found that DC precursors from peripheral blood after mobilization produced DC showed a high quantity of production and higher purity, and to be fully functioned. Conclusions DC precursors from peripheral blood after mobilization were a good source of DC. |
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| Keywords: | dendritic cells costimulatory molecule naive T cells cytokine |
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