C-erbB-2、P~(53)和PCNA在前列腺癌中的表达及其意义

目的研究前列腺癌及前列腺增生症组织中C-erbB-2和P53PCNA表达的关系及其临床意义。方法采用免疫组织化学方法检测31例前列腺增生及69例前列腺癌患者C-erbB-2、P53和PCNA的表达水平。结果①C-erbB-2和P53在前列腺癌的阳性率分别是56.52%和69.56%,均高于良性前列腺增生的阳性率,但差异无统计学意义(P>0.05),②在前列腺癌中,低分化组C-erbB-2的表达水平明显高于高、中分化组,差异有统计学意义(P<0.05);③P53表达水平在上述各组之间差异均有统计学意义(P<0.05);④PCNA在前列腺癌组织中表达较前列腺良性增生症组织中增加,差异有统计学意义(P<0.001);PCNA的表达随着组织学分级及临床分期的增加而增加(P<0.001),PCNA高表达组术后生存率明显低于低表达组(P<0.01)。结论P53蛋白表达与肿瘤的分级和分化无统计学意义,而前列腺癌C-erbB-2和PCNA蛋白的表达异常增高,可能与前列腺癌的发生发展有关,联合检测PCNA和C-erbB-2对其肿瘤的恶性程度、生物学行为及预后评估有着重要意义。

Study on the correlation between C-erbB-2.P53 and PCNA expression In human prostate carcinoma

Objective To study the correlation between and C-erbB-2 P53 PCNA expression in prostatic carcinoma and benign prostatic hyperplasia(BPH)as well as their clinical significance.Methods The expression of C-erbB-2,P53,PCNA proteins in sixty-nine cases of prostatic carcinoma and 20 case benign prostatic hyperplasia were detected by immunohistochemical methods.Results ① Expression rates of C-erbB-2 of and P53 in prostatic carcinoma were 56.52% and 69.56%.Both of which were not significantly higher than those in benign hyperplasia of prostatie statically;② the expression of C-erbB-2 in poor differentiation group was significantly higher than that in well differentiation group(P<0.05);③ P53 expression in prostatic carcinoma has statistically significant difference;④The expression of PCNA in carcinoma tissue was significant higher than that in the benign prostatic hyperplasia tissue,the difference was significant(P<0.01).The expression of PCNA increased with the increase of pathological grade and clinical grade(P<0.01).There was a significant difference in survival rates between PCNA high expression group and low expression group.(P<0.01).Conclusion P53 expression between different group was no statistically significant difference.High expression of C-erbB-2 and PCNA in human prostatic carcinoma might involve in the pathogenesis of the disease.C-erbB-2 and PCNA protein assay can be used as an index of diagnosis and prognosis of the disease.