Human osteoblast damage after antiseptic treatment |
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Authors: | Pauline Vörös Oliver Dobrindt Carsten Perka Christoph Windisch Georg Matziolis Eric Röhner |
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Affiliation: | .Department of Traumatology and Orthopaedics, Charité University Hospital, Berlin, Germany ;.Department of Orthopaedics, Rudolf-Elle-Hospital, Friedrich-Schiller-University, Jena, Germany ;.Orthopaedic Department, Friedrich-Schiller-University, Campus Eisenberg Klosterlausnitzer Straße 81, 07607 Eisenberg, Germany |
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Abstract: | Purpose Antiseptics are powerful medical agents used for wound treatment and decontamination and have a high potential for defeating joint infections in septic surgery. Both chlorhexidine and polyhexanide are frequently used in clinical practice and have a broad antimicrobial range, but their effect on human osteoblasts has not been sufficiently studied. Our objective was to investigate the toxic effects of polyhexanide and chlorhexidine on human osteoblasts in vitro to evaluate their clinical applicability in septic surgery. Methods We isolated and cultivated human osteoblasts in vitro and assayed the toxic effects of chlorhexidine 0.1 % and polyhexanide 0.04 %, concentrations commonly applied in clinical practice. Toxicity analysis was performed by visualisation of cell structure, lactate dehydrogenase (LDH) activity and evaluation of vital cells. Toxicity was evaluated by microscopic inspection of cell morphology, trypan blue staining and determination of LDH release. Results Damaged cell structure could be shown by microscopy. Both antiseptics promoted LDH activity after incubation with osteoblasts. The evaluation of vital osteoblasts showed a significant decrease of vital cells. Conclusions Both antiseptics induced significant cell death of osteoblasts at optimum exposure. We therefore recommend cautious use of polyhexanide and chlorhexidine in septic surgery to avoid severe osteoblast toxicity. |
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Keywords: | Human osteoblasts in vitro Polyhexanide Chlorhexidine Periprosthetic joint infections |
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