木贼对动脉粥样硬化早期大鼠内皮细胞凋亡及Bax、Bcl-2表达的影响

目的 观察木贼对大鼠动脉粥样硬化早期血管内皮细胞凋亡及bax、bcl—2表达的影响。方法 高脂饲料复制SD大鼠高脂血症和早期动脉粥样硬化模型，同时给以不同剂量木贼水煎刑灌胃，实验10w后，流式细胞术定量检测各组主动脉内皮细胞凋亡率及bax、bcl—2基因蛋白表达。结果 模型组内皮细胞凋亡率及两个基因表达明显高于正常组(P＜0．01)；木贼组内皮细胞凋亡率与bax表达量显著低于模型组(P＜0．01)，bcl—2比较虽无统计学意义，但用药后bax／bcl—2比值差异具有显著性。结论 木贼可从基因水乎调控早期动脉粥样硬化主动脉内皮细胞凋亡，干预动脉粥样硬化始动环节及其发生。

Effects of Equisetum on the apoptosis of early atherosclerosis in rats' aorta endothelium

Objective To investigate the effect of Equisetum that controlling the apoptosis of aorta endothelium by regulating the expression of Bax and Bcl-2 on experimental hypercholesterolemia and atherosclerosis.Methods Forty-eight Sprague-Dawley rats were randomly divided into four groups: a normal group with standard chow diet, and three other groups fed with a high fat diet including two groups plus Equisetum at different doses. The rate of apoptosis and the expressions of Bax and Bcl-2 in aorta endothelium were detected by the way of flow cytometry after ten weeks.Results The rate of apoptosis of endothelium in model group was higher than those in normal group (P<0.01). The expressions of Bax were enhanced (P<0.01). The rate of apoptosis ?the expression of Bax and the amount of Bax/Bcl-2 in Equisetum groups were decreased compared with those in model group (P<0.01).Conclusions Equisetum appear action on regulating the apoptosis of aorta endothelium by regulating the expression of Bax .