大鼠肝细胞线粒体周围内质网的三维重建

目的：研究肝细胞线粒体与内质网的空间结构关系。 方法：通过连续超薄切片重建线粒体与其周围内质网的三维模型，结果：连续超薄切片和三维模型显示：除极少量的线粒体与脂滴直接接触外，几乎每个线粒体都被内质网所包裹，大多数线粒体周围既有RER又有小囊泡状SER，少数线粒体则仅有小的SER。这些内质网与线粒体紧贴如同线粒体外壳，二者的间隙小于30nm,其间无任何其它细胞器。结论：肝细胞线粒体通常在内质网包绕下执行生理功能，二者形成的结构和功能紧系的整体。

Three-dimensional reconstruction of the endoplasmic reticulum binding mitochondria in rat liver cell

Objective To study the three dimensional structural relationship between mitochondria and endoplasmic reticulum (rough endoplasmic reticulum,RER and smooth endoplasmic reticulum, SER). Methods Three dimensional models of mitochondria and the endoplasmic reticulum binding mitochondria in rat liver cells were reconstructed by observing serial ultrathin sections in EM. Results Every mitochondrion was bound with endoplasmic reticulum(SER and RER) except that mitochondria contacted with fat drop directly.Most mitochondria were in part capped with RER or small vesicles of SER,Which seemed at place to form a cap around the mitochondria.There were very small space(<30nm)without any organelles between the mitochondria and endoplasmic reticulum binding the mitochondria. Conclusion The mitochondria were bound with endoplasmic reticulum (SER and RER) when they took action in liver cells.Mitochondria and endoplasmic reticulum binding the mitochondria is a unity in structure and function.