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Nanofibrous nerve conduits for repair of 30-mm-long sciatic nerve defects* |
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| Authors: | Esmaeil Biazar Saeed Heidari Keshel Majid Pouya Hadi Rad Melody Omrani Nava Mohammad Azarbakhsh Shirin Hooshmand |
| Institution: | Esmaeil Biazar (Department of Biomaterial Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran)； Saeed Heidari Keshel (Student Research Committee, Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran)； Majid Pouya (Department of Medical Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran)； Hadi Rad (Department of Medical Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran)； Melody Omrani Nava (Department of Medical Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran)； Mohammad Azarbakhsh (Faculty of Medical Sciences, Babol University of Medical Sciences, Babol, Iran)； Shirin Hooshmand(Faculty of Medical Sciences, Babol University of Medical Sciences, Babol, Iran)； |
| Abstract: | It has been confirmed that nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nerve conduit can promote peripheral nerve regeneration in rats. However, its efficiency in repair of over 30-mm-long sciatic nerve defects needs to be assessed. In this study, we used a nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nerve conduit to bridge a 30-mm-long gap in the rat sciatic nerve. At 4 months after nerve conduit implantation, regenerated nerves were macroscopi-cal y observed and histological y assessed. In the nanofibrous graft, the rat sciatic nerve trunk had been reconstructed by restoration of nerve continuity and formation of myelinated nerve fiber. There were Schwann cel s and glial cel s in the regenerated nerves. Masson’s trichrome staining showed that there were no pathological changes in the size and structure of gastrocnemius muscle cel s on the operated side of rats. These findings suggest that nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nerve conduit is suitable for repair of long-segment sciatic nerve defects. |
| Keywords: | neural regeneration peripheral nerve injury sciatic nerve artificial conduit nanofiber poly(3-hydroxybutyrate-co-3-hydroxyvalerate) macroscopic observation histology grants-supported paper neuroregeneration |
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