Basic fibroblast growth factor attenuates the degeneration of injured spinal cord motor endplates** |
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| Authors: | Jianlong Wang Jianfeng Sun Yongxiang Tang Gangwen Guo Xiaozhe Zhou Yanliang Chen Minren Shen |
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| Affiliation: | Jianlong Wang (Department of 0rthopedics, Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China); Jianfeng Sun(Department of 0rthopedics, Yichang Central People’s Hospital, Yichang 443003, Hubei Province, China);Yongxiang Tang(Department of Nuclear Medicine, Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China);Gangwen Guo(Department of 0rthopedics, Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China);Xiaozhe Zhou(Department of 0rthopedics, Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China);Yanliang Chen(Department of 0rthopedics, Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China);Minren Shen(Department of 0rthopedics, Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China); |
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| Abstract: | The distal end of the spinal cord and neuromuscular junction may develop secondary degeneration and damage fol owing spinal cord injury because of the loss of neural connections. In this study, a rat model of spinal cord injury, established using a modified Al en’s method, was injected with basic fibroblast growth factor solution via subarachnoid catheter. After injection, rats with spinal cord injury displayed higher scores on the Basso, Beattie and Bresnahan locomotor scale. Motor function was also wel recovered and hematoxylin-eosin staining showed that spinal glial scar hyperplasia was not apparent. Additional y, anterior tibial muscle fibers slowly, but progressively, atrophied. Immunohistochemical staining showed that the absorbance values of calcitonin gene related pep-tide and acetylcholinesterase in anterior tibial muscle and spinal cord were similar, and injection of basic fibroblast growth factor increased this absorbance. Results showed that after spinal cord injury, the distal motor neurons and motor endplate degenerated. Changes in calcitonin gene related pep-tide and acetylcholinesterase in the spinal cord anterior horn motor neurons and motor endplate then occurred that were consistent with this regeneration. Our findings indicate that basic fibroblast growth factor can protect the endplate through attenuating the decreased expression of calcitonin gene related peptide and acetylcholinesterase in anterior horn motor neurons of the injured spinal cord. |
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| Keywords: | neural regeneration spinal cord injury motor endplate basic fibroblast growth factor calcitonin gene related peptide acetylcholinesterase subarachnoid catheter grants-supported paper neu-roregeneration |
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