甲基苯丙胺致大鼠神经毒性及纹状体硝化作用的改变

目的探讨甲基苯丙胺(methamphetamine,MA)所导致的大鼠神经毒性及纹状体硝化作用的改变。方法建立MA给药模型,对照组给予等量生理盐水;观察两组大鼠的行为学改变,体温变化,纹状体中NO含量,NOS活性和蛋白质硝化水平的变化情况。结果MA组大鼠刻板行为评分和体温均高于对照组(P<0.01);与对照组相比,MA组大鼠纹状体NO含量及NOS活性增加(P<0.01);纹状体蛋白硝化水平明显增加(P<0.01),差异有显著性。结论MA可导致大鼠行为学改变和体温变化,大鼠脑组织NO含量、NOS活性及蛋白硝化水平的增高可能是MA中枢神经系统损伤的重要机制。

Methamphetamine neurotoxicity and changes of nitration in rats striatum

Aim To investigate the neurotoxicity and the changes of nitration in the striatum of rats treated with methamphetamine(MA).Methods The rats were randomly divided into MA group and control group.The stereotyped behavior and body temperature of the rats were recorded.The differences of nitric oxide, nitric oxide synthase(NOS)and nitroprotein were compared between the two groups.Results The score of stereotyped behavior and the body temperature of the rats in MA group were significantly higher than those in the control group(P ＜0.01);Compared with the control group, the content of NO and the activity of NOS of striatum in MA group were significantly increased(P ＜0.01), and nitration of protein was also upregulated(P ＜0.01).Conclusion The increases of NOS activity, NO level and nitroprotein may play an important role in MA-induced neurutoxity in rat striaturn.