| 高效液相色谱法测定染色黄芩中金胺的含量 |
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| 引用本文： | 莫连峰,饶伟文,赵纯玉. 高效液相色谱法测定染色黄芩中金胺的含量[J]. 中国药业, 2007, 16(15): 29-30 |
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| 作者姓名： | 莫连峰 饶伟文 赵纯玉 |
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| 作者单位： | 广西壮族自治区桂林食品药品检验所,广西,桂林,541002 |
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| 摘 要： | 目的 探讨以高效液相色谱（HPLC）法测定染色黄芩中化工染料金胺的含量.方法 采用Luna C18柱（250mm ×4.6 mm,5μm）为色谱柱,流动相为0.025 mol/L磷酸盐缓冲液（磷酸二氢钾3.402 g,加水至1 000 mL,内加0.2%三乙胺,并用磷酸调节pH值至3.0）-乙腈（65∶35）,流速为1.0 mL/min,检测波长为432 nm.结果 金胺质量浓度线性范围为5～300 μg/mL,r=0.999 9（n=10）,平均回收率为99.39%（n=9）,RSD=0.23%.结论 HPLC法简便、快速、准确.
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| 关 键 词： | 高效液相色谱法 金胺 黄芩 |
| 文章编号： | 1006-4931（2007）15-0029-02 |
| 修稿时间： | 2006-11-152007-03-07 |
Quantitative Determination of Dyed Auramine in Radix Scutellariae by HPLC |
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| Mo Lianfeng,Rao Weiwen,Zhao Chunyu. Quantitative Determination of Dyed Auramine in Radix Scutellariae by HPLC[J]. China Pharmaceuticals, 2007, 16(15): 29-30 |
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| Authors: | Mo Lianfeng Rao Weiwen Zhao Chunyu |
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| Affiliation: | Guangxi Guilin Institute for Food and Drug Control, Guilin, Guangxi, China 541002 |
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| Abstract: | Objective To establish a HPLC method for the quantitative determination of the dyed auramine in radix scutellariae. Methods The separation was performed on a LUNA C18 column （250 mm×4. 6 ram,5 μm）, with the mixture （35 ：65） of acetonitrile-phosphate buffer （dissolved 3. 402 g of KH2PO4 in 1 000 mL water, added 0.2% triethylmine, adjusted pH value to 3.0 with H3PO4）. The flow rate was 1.0 mL/min. The UV detection wavelength was set at 432 nm. Results A good linearity was obtained from 5-300 μg/mL, r =0. 999 9 （ n = 10）. The average recovery rate was 99.39% （ n = 9）, RSD = 0. 23%. Conclusion The method is simple, rapid and accurate. |
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| Keywords: | HPLC auramine radix scutellariae |
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