人巨细胞病毒皮层蛋白pUL23与宿主蛋白IGFBP4相互作用的鉴定

目的:利用蛋白质相互作用的技术筛选与pUL23相互作用宿主蛋白,为研究pUL23蛋白对人巨细胞病毒繁殖的影响提供线索。方法:通过酵母双杂交系统从人胚肾cDNA文库筛选与pUL23相互作用的宿主蛋白;通过GST-pu ll-down技术研究二者体外物理性相互作用;免疫共沉淀技术进一步研究二者在胞内相互作用。结果:Pu ll-down技术、免疫共沉淀技术确定了宿主蛋白IGFBP4与pUL23具有相互作用。结论:上述结果为研究pUL23蛋白调节病毒自身繁殖功能提供重要依据。

Identification of human cytomegalovirus tegument protein pUL23 interacting with human IGFBP4

AIM: pUL23 is a protein encoded by human cytomegalovirus, which inhibits self propagation of the virus. The study was to investigate the function of pUL23 protein encoded by human cytomegalovirus and reveal the mechanism which inhibits propagation of the virus itself.METHODS: We screened a host proteins interacting with pUL23 from human embryonic kidney cDNA library using the yeast two-hybrid assay. GST-pull-down and immunoprecipitation techniques were applied to study the physical interaction between them in vitro and in vivo, respectively.RESULTS: We confirmed the interaction between pUL23 and IGFBP4 by pull-down and immunoprecipitation technology.CONCLUSION: These results might provide important information to the function of pUL23 in regulating self-reproducting.