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| 毒死蜱致大鼠空间记忆损伤及ERK/CREB信号途径抑制 | |
| 引用本文： | 闫长会,焦利飞,赵君,阳海鹰,彭双清.毒死蜱致大鼠空间记忆损伤及ERK/CREB信号途径抑制[J].军事医学科学院院刊,2012,36(5):341-344. |
| 作者姓名： | 闫长会 焦利飞 赵君 阳海鹰 彭双清 |
| 作者单位： | 1. 军事医学科学院疾病预防控制所,北京,100071;军事医学科学院毒物药物研究所,北京,100850 2. 军事医学科学院疾病预防控制所,北京,100071 3. 军事医学科学院毒物药物研究所,北京,100850 |
| 基金项目： | 国家自然科学基金资助项目（30800929） |
| 摘 要： | 目的探讨有机磷杀虫剂毒死蜱（chlorpyrifos,CPF）长期染毒对大鼠空间记忆的影响及其可能机制。方法选择不引起大鼠出现全身系统毒性的CPF剂量（1,5,10 mg/kg）对大鼠连续染毒12周,利用Morris水迷宫评价大鼠的空间记忆功能,利用Western印迹检测CREB信号通路的蛋白表达。结果 CPF染毒大鼠到达平台的潜伏期呈剂量依赖性增加;随着染毒剂量的增加,大鼠海马CREB及ERKⅠ/Ⅱ磷酸化蛋白逐渐减少,而CaMKⅡ磷酸化蛋白、PKA蛋白表达没有明显改变。结论 CPF长期染毒能够诱发大鼠空间记忆的损伤,ERK/CREB信号通路的抑制可能是参与该变化的重要信号途径。 |
| 关 键 词： | 毒死蜱 空间记忆 cAMP反应单元结合蛋白 细胞外调节蛋白激酶 |
Chlorpyrifos caused spatial memory impairment and inhibition of the ERK/CREB signal system |
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| YAN Chang-hui , JIAO Li-fei , ZHAO Jun , YANG Hai-ying , PENG Shuang-qing.Chlorpyrifos caused spatial memory impairment and inhibition of the ERK/CREB signal system[J].Bulletin of the Academy of Military Medical Sciences,2012,36(5):341-344. | |
| Authors: | YAN Chang-hui JIAO Li-fei ZHAO Jun YANG Hai-ying PENG Shuang-qing |
| Institution: | 1. Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China; 2. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China) |
| Abstract: | Objective To evaluate the changes in spatial memory and CREB signal systems involved in the repeated ex- posure to chlorpyrifos （CPF） in rats. Methods The CPF （0,1.0, 5.0 or 10.0 mg/kg） was administered daily to male Wistar rats for 12 weeks. Morris water maze was used to evaluate the spatial memory function and the Western blot was used to analyze the protein expression. Results The CPF treated rats took more time to reach the platform in the water maze test. The phosphorylated protein expression of ERK I / II and CREB decreased. Conclusion Chronic exposure to CPF could result in spatial memory impairment. The inhibition of ERK/CREB signal system may be the important pathway to regulate the CPF caused spatial memory deficit. |
| Keywords: | chlorpyrifos spatial memory CREB ERK1/2 |
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