混合脐血血浆对脐血CD34^＋细胞体外扩增的作用

采用两步法分离出脐血CD34~ 细胞,比较研究了混合脐血血浆联合IL-3,IL-6,GM-CSF,Epo 4种中、晚期造血因子和单纯的4种造血因子情况下脐血CD34~ 细胞的体外扩增。结果表明,混合脐血血浆联合造血因子对粒-巨噬细胞集落形成单位(granulocyte-macrophage colony-forming unit,CFU-GM),红系爆式集落形成单位(burst-forming unit of erythriod,BFU-E),混合集落形成单位(minxed colony-forming unit,CFU-mix)3种集落的扩增效果明显优于单纯的4种造血因子联合组,差异具有统计学意义(P<0.01),但单纯混合脐血血浆扩增效果较差。脐血造血细胞扩增对子成人脐血移植有重要意义。上述结果提示,混合脐血血浆的扩增成功,可代替或弥补早期造血生长因子的作用,用于脐血造血细胞的体外扩增。

Effect of mixed umbilical cord blood plasma on ex vivo expansion of CD34~ cells from umbilical cord blood

Using two-step-adhering method, we separated CD34 cells from human umbilical cord blood and compared the expanding effect of CD34 cells from mixed umbilical cord blood plasma combined with IL-3, IL-6, GM-CSF, Epo and pure combination of IL-3, IL-6, GM-CSF, Epo. The results indicated that the expanding effect of mixed umbilical cord blood plasma combined with four kinds of hematopoietic growth factors on CFU-GM, BFU-E, CFU-mix was more superior than that of pure combination of four kinds of hematopoietic growth factors. The difference has statistical value (P<0.01), but the expanding effect of pure mixed umbilical cord blood plasma is poor. In vitro expansion is important to adult in umbilical cord blood transplantation. The successful expansion of CD34 cells from mixed umbilical cord blood plasma showed that mixed umbilical cord blood plasma may replace or compensate the effect of earlier hematopoietic growth factors in vitro expansion.