| 血小板生成素是人红白血病HEL细胞自分泌因子的证据 |
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| 引用本文： | 张晓泉,杨耀明,陆爱丽,侯纬敏,王申五,王德炳. 血小板生成素是人红白血病HEL细胞自分泌因子的证据[J]. 中国实验血液学杂志, 1998, 6(1): 14-18 |
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| 作者姓名： | 张晓泉 杨耀明 陆爱丽 侯纬敏 王申五 王德炳 |
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| 作者单位： | 北京医科大学人民医院血液病研究所，北京医科大学人民医院血液病研究所，北京医科大学生物化学系，北京医科大学生物化学系，北京医科大学人民医院血液病研究所，北京医科大学人民医院血液病研究所 北京 100044，北京 100044，北京 100083，北京 100083，北京 100044，北京 100044 |
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| 基金项目： | 高等学校博士学科点专项科研基金 编号9501008 |
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| 摘 要： | 为了研究人红白血病HEL细胞有自分泌血小板生成素(Tpo)的能力,采用细胞原位杂交检测mRNA,采用免疫荧光方法、生物素亲和素系统检测蛋白质,采用细胞传感器检测细胞电化学行为。结果表明:Mpl是细胞膜受体;HEL细胞内存在Tpo蛋白质;在培养基中加入rhTpo后HEL细胞增殖;可溶性受体融合蛋白可以阻断HEL细胞增殖,阻断后HEL细胞的电子传递数减少,但细胞数高于接种细胞数。实验结论提示Tpo可能是HEL细胞的自分泌因子。
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| 关 键 词： | 血小板生成素 HEL细胞 Mpl 自分泌因子 |
Thrombopoietin is a possible autocrine growth factor of HEL cell line |
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| ZHANG Xiao-Quan YANG Yao-Ming LU Ai-Li HOU Wei-MinWANG Shen-Wu WANG De-Bing. Thrombopoietin is a possible autocrine growth factor of HEL cell line[J]. Journal of experimental hematology, 1998, 6(1): 14-18 |
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| Authors: | ZHANG Xiao-Quan YANG Yao-Ming LU Ai-Li HOU Wei-MinWANG Shen-Wu WANG De-Bing |
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| Abstract: | In order to identify Tpo as the autocrine growth factor of human erythroleukemic cell line (HEL cells). non-radioactive in situ hybridization was used to detect mRNAs of Tpo and c-mpl, indirect immunofluorescence and biotin-avidin system were used to detect Tpo and Mpl, a cytosensor was used to analyze the electronic voltammetric behavior (EVB) of HEL cells. Results indicated: Mpl as a membrane receptor on HEL cells, Tpo protein existing in HEL cells, rhTpo promoting proliferation of HEL cells and Mpl-IgG blocking the proliferation of HEL cells. After the Mpl-IgG blocking, EVB of HEL cells decreased, but there was still a discordance between cell count and EVB of HEL cells. It was concluded that Tpo may be a possible autocrine factor of HEL cells. |
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| Keywords: | thrombopoietin HEL cell Mpl autocrine factor |
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