结核分枝杆菌荧光定量PCR检测方法的建立与应用

目的建立结核分枝杆菌的荧光定量PCR检测方法，探讨荧光PCR检测痰标本中结核分枝杆菌的应用价值。方法根据结核分枝杆菌基因保守序列设计引物和探针，并构建标准品。对102例培养涂阳的结核痰液标本和45例非结核感染者的痰标本，应用荧光定量PCR法、痰涂片抗酸染色法检测结核分枝杆菌。结果荧光定量PCR、抗酸染色法检测结核分枝杆菌的特异性分别为100％、30％。结论荧光定量PCR是一种快速、敏感性高、特异性强的结核分枝杆菌辅助诊断方法，具有重要的应用价值。

Establishment and application of fluorescence quantitative PCR for detection of Mycobacterium tuberculosis

Objective To constitute a method for detection of Mycobacterium tuberculosis by fluorescence quantitative PCR and evaluate the application value of fluorescence quantitative PCR in detection of Mycobacterium tuberculosis in sputum.Methods The primers and probe were designed and constitute a standards.The suptum samples from 102 tuberculosis patients and 45 non-tuberculosis patients were detected by fluorescence quantitative PCR and acid-fast stain.Results The positive rates and specificity of fluorescence quantitative PCR,acid-fast stain,were100% and 30% respectively.Conclusion Fluorescence quantitative PCR is a rapid method for diagnosis of tuberculosis,which shows a high specificity and sensitivity.It is a useful tool for diagnosis of tuberculosis in the future.