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梅毒螺旋体不同血清学检验方法的应用评价
引用本文:黄宏鑑,蒋翡翎,李春芸,陆玉珠. 梅毒螺旋体不同血清学检验方法的应用评价[J]. 中国热带医学, 2006, 6(1): 131-131,169
作者姓名:黄宏鑑  蒋翡翎  李春芸  陆玉珠
作者单位:1. 海口市人民医院,海南,海口,570208
2. 海南省皮肤性病防治研究中心,海南,海口,570000
摘    要:目的评价酶联免疫吸附试验(ELISA)、梅毒螺旋体明胶颗粒凝集试验(TPPA)和环状卡片快速血浆反应素试验(RPR)对梅毒病人诊断的敏感性和特异性。方法使用目前最为常用的梅毒螺旋体感染诊断ELISA和TPPA及RPR试剂对60例梅毒病人血清进行检测。结果ELISA法的阳性率96.67%,TPPA法阳性率98.33%,RPR法66.67%。结论ELISA法是一种高特异性,高敏感性的梅毒血清学诊断检测方法。

关 键 词:梅毒血清学诊断  螺旋体明胶颗粒凝集试验(TPPA):酶联免疫吸附试验(ELISA)  环状卡片血浆反应素试验(RPR)
文章编号:1009-9727(2006)01-131-01
收稿时间:2005-10-20
修稿时间:2005-10-20

Clinical evaluation of different serological methods for diagnosis of Treponema pallidum
HUANG Hong - jian,JIANG Fei - ling, LI Chun - yun, et al,. Clinical evaluation of different serological methods for diagnosis of Treponema pallidum[J]. China Tropical Medicine, 2006, 6(1): 131-131,169
Authors:HUANG Hong - jian  JIANG Fei - ling   LI Chun - yun   et al  
Abstract:Objective To evaluate the sensitive and accuracy of ELISA, TPPA and RPR methods in diagnosis of treponemal infection. Methods The serun from 60 syphilis patienes were detected by ELISA, TPPA and RPR methods. Results The positiverate detected by ELISA, TPPA and RPR methods were 96.67%, 98.33% and 66.67%. Conclusion ELISA was evidented with a high sensitive and a high specific serological method in diagnosis of treponemal infection.
Keywords:Syphilis serodiagnosis   TPPA    Enzyme linked immunoadsorbent assay   Rapid plasma reagintest
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