155例非霍奇金淋巴瘤患者细胞遗传学分析

目的 了解非霍奇金淋巴瘤（NHL）患者染色体异常与WHO病理组织分型之间的关系，并与国外NHL染色体异常类型进行比较。方法 采用常规染色体G带分析和荧光原位杂交（FISH）方法对155例NHL患者的淋巴结组织进行细胞和分子遗传学研究。结果 155例NHL患者中常见的病理类型是弥漫大B细胞淋巴瘤（DLBCL）（59例，38．1％）、滤泡性淋巴瘤（27例，17．4％）、B小淋巴细胞淋巴瘤（16例，10．3％）、非特指周同T细胞淋巴瘤（13例，8．4％）、血管免疫母细胞性T细胞淋巴瘤（11例，7．1％）。155例NHL患者中染色体异常为119例，占76．8％。滤泡性淋巴瘤、B小淋巴细胞淋巴瘤、DLBCL、间变性大细胞淋巴瘤和前体T淋巴母细胞淋巴瘤染色体异常率较高，分圳为96．3％、87．5％、86．4％、83．3％、83．3％。DLBCL中复杂核型占86．3％，染色体结构异常累及最多的是3，6，14，1号染色体，41．2％为3q27异常，43．1％的病例有1号染色体异常。6q21、6q23和6q25异常占23．5％。DLBCL中典型t（14；18）的病例只有2例，明湿低于国外报道。用FISH方法检测DLBCL中IgH重排阳性率为40．1％。16例B小淋巴细胞淋巴瘤均未发现13q14缺失，只发现2例有13q10异常。11例血管免疫母细胞性T细胞淋巴瘤中只有3例核型异常。结论 我国淋巴瘤的病理类型分布与欧美国家有明显不同。尽管DLBCL染色体异常类型基本与国外相似，但t（14；18）较少见。与国外报道相比，B小淋巴细胞淋巴瘤和血管免疫母细胞性T细胞淋巴瘤染色体异常率较低，染色体异常类型也有差异。

Cytogenetic study on 155 cases of non-Hodgkin's lymphoma

Objective To investigate the relationship between histopathological subtype of non- Hodgkin's lymphoma(NHL) and chromosomal abnormalities,and compare the difference of chromosomal ab- normalities between China and the West.Methods Routine G banding chromosome analysis and fluorescent in situ hybridization(FISH)were performed on lymph node specimens from 155 NHL patients.Results Dill'use large B-cell lymphoma(DLBCL)constituted 38.1% of the cases followed by follicular lymphoma(FL) 17.4% ,small lymphocytic lymphoma(SLL) 10.3% ,peripheral T-cell lymphoma(PTCL)(unspecified) 8.4% ,and angioimmunnblastic lymphoma 7.1%.One hundred nineteen patients (76.8%) had clonal chro- mosomal abnormalities.The incidence of chrnmosomal abnormalities among FL,SLL,DLBCL,anaplastic large cell lymphoma (ALCL) and precursor T-cell lymphohlastie lymphoma (TLBL) was 96.3% ,87.5%, 86.4% ,83.3% and 83.3% ,respectively.Complex karyotype was 86.3% in DLBCL.The most frequent structural abnormalities in DLBCL involved ehromosomes 3,6,14 and 1,with had high frequencies of 3q27 (41.2%) and 6q21 ,6q23,6q25 involvement (23.5%).But only 2 cases of DLBCL had classical t (14; 18) (q32;q21) which was lower than that in the West (20%).The positive rate of IgH rearrangement was 40.1% in DLBCL by FISH.No 13q14 deletion was found in 16 cases of SLL.Normal karyotype was observed in 8/11 cases with angioimmunoblastic T-cell lymphoma patients.Conclusions The distribution of the his- topathologieal subtypes of NHL is different among different geographical areas.The chromosomal abnormalities in DLBCL was comparable between China and the West,but t(14;18)was infrequent in the tbrmer.The chromosomal abnormalities in SLL was different from the West.The incidence of chromosomal abnormalities in angioimmunoblastie T-cell lymphoma was lower than that in the West.