| CT引导下经皮穿刺气管内注射平阳霉素致大鼠肺纤维化模型的建立及其病理演变 |
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| 引用本文： | 孙鸿,李春平,李睿,陈果.CT引导下经皮穿刺气管内注射平阳霉素致大鼠肺纤维化模型的建立及其病理演变[J].西部医学,2011,23(4):617-620. |
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| 作者姓名： | 孙鸿 李春平 李睿 陈果 |
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| 作者单位： | 1. 川北医学院附属医院,四川,南充637000;仪陇县人民医院,四川,仪陇,637600
2. 川北医学院附属医院,四川,南充637000 |
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| 摘 要： | 目的探讨CT引导下经皮穿刺气管内注射平阳霉素诱导大鼠肺纤维化的可行性。方法 60只成年健康SD大鼠随机分为对照组和实验组（3d、7d、14d、28d、56d）,每组10只。大鼠固定、麻醉后,CT引导下用一次性无菌注射器经颈部穿刺气管,穿刺成功后,实验组向气管内注射5mg/ml平阳霉素溶液1ml/kg,对照组注入生理盐水1ml/kg。注药后3d、7d、14d、28d、及56d,对双肺标本行HE染色及Masson染色,在每张组织切片上对肺泡炎和肺纤维化进行半定量评分。结果实验组大鼠肺泡炎和肺纤维化明显较对照组严重,实验组炎症评分以7d组最高（11.53±2.81,P〈0.01）,纤维化评分以56d组最高（7.06±1.69,P〈0.01）。结论 CT引导下经皮穿刺气管内注射平阳霉素复制大鼠肺纤维化模型是切实可行的。
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| 关 键 词： | 肺纤维化 动物模型 体层摄影术 X线计算机 经皮穿刺术 |
Establishment and pathological evolution of a rat model of pulmonary fibrosis by CT-guided percutaneous punctures intratracheal instillation of peplomycin |
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| SUN Hong,LI Chun-ping,LI Rui,et al.Establishment and pathological evolution of a rat model of pulmonary fibrosis by CT-guided percutaneous punctures intratracheal instillation of peplomycin[J].Medical Journal of West China,2011,23(4):617-620. |
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| Authors: | SUN Hong LI Chun-ping LI Rui |
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| Institution: | SUN Hong1,2,LI Chun-ping1,LI Rui1,et al |
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| Abstract: | Objective To establish a rat model of pulmonary fibrosis by CT-guided percutaneous punctures intratracheal instillation of peplomycin and evaluate the model by histopathology.Methods60 healthy Sprague-Dawley rats were randomly divided into experimental group(3d,7d,14d,28d and 56d) and control group.10 rats were in each group.After the rats were anesthetized and fixed,CT-guided percutaneous punctures of trachea were conducted with a abacterial syringe.The experimental group were given intratracheally 5mg/ml ... |
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| Keywords: | Pulmonary fibrosis Animal model X-ray computed tomography Percutaneous punctures |
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