枳术丸口服给药后血中移行成分分析及其定量研究

目的：确认枳术丸中有效成分并建立其含量测定方法。方法：应用血清药物化学的方法，对口服枳术丸后血清中移行成分进行分析，并对主要血清中成分在相应的最大吸收波长进行含量测定。结果：口服给药后，大鼠血清中检测到13个化合物，其中6个为枳术丸中原型成分，7个为代谢产物；并建立了枳术丸中入血成分柚皮苷、橙皮苷的含量测定方法，其二个成分的加样回收率分别为101．13％、98．56％。结论：柚皮苷、橙皮苷及新橙皮苷等为枳术丸主要体内直接作用物质，所建立枳术丸中有效成 分测定方法能够全面、准确地控制枳术丸的内在质量。

A Qualitative & Quantitative Analysis: Migrating Constituents of Zhizhu Pills in Rat Blood

The aim of this analysis is to determine the active compounds of Zhizhu Pills in vivo, and establish an approach to measure the contents using HPLC. Serum pharmacochemistry and RP - HPLC were used to measure the concentration of two main constituents in the oral pills, at a wavelength of 283nm. 13 compounds were detected in the rat blood, after an oral administration of Zhizhu Pills. 6 of them were original in form, and 7 others were metabolites. An approach was created to measure Hesperidin and Naringin, with a respective recovery of 101.13% and 98.56%. It is concluded that Hesperidin, Neohesperidin and Naringin are the main elements that have a direct effect. The established approach is able to stage a comprehensive and accurate quantitative analysis of Zhizhu Pills.