KSC联合紫杉醇对人肝癌HepG-2细胞株的抑制作用研究

目的:研究硒酸酯多糖(KSC)与紫杉醇(TAX)联合对肝癌细胞株HepG-2的生长抑制作用,判断联合用药的效果。方法:采用MTT法测定药物的体外杀伤作用,应用金氏公式进行联合用药分析。结果:KSC与TAX单用均可抑制HepG-2细胞的生长,且呈时间—剂量依赖性,二者合用后对HepG-2细胞的抑制作用显著增强。两药合用的中效浓度分别为31.684mg/L和0.272nmol/L,较两药单用时的中效浓度低(KSC单用为46.632mg/L,TAX单用为1.684nmol/L)。两药合用的绝大多数q值在0.882～0.989,为相加作用。结论:KSC与TAX联用可显著增强对HepG-2细胞的抑制作用,二者具有协同相加抗肿瘤作用。

Effect of Combination of Kappa-selenocarrageenan and Paclitaxel on Human Hepatoma HepG-2 Strain

Objectlve ：To study the interaction of kappa-selenocarrageenan （KSC）and paclitaxel （TAX）on human hepatocellular carcinoma HepG-2 in vitro in order to judge the effect of combination.Methods：The MTT was used to estimate the killing effect of drugs and the King＇s principle was used to analyze union of medication.Results：KSC and TAX could respectively inhibit HepG-2 cells proliferation in a time and concentration dependent patterns ,which was enhanced significantly as combined with each other.The medianeffect concentrations （IC50）of KSC and TAX in combination were 31.684mg/L and 0.272nmol/L,which was tess than that of single use of KSC （46.632mg/L）and TAX （1.684nmol/L）.The q values of most combinations were from 0.882 to 0.989 and they acted in an additive pattern.Conclusion： KSC combined with TAX can significantly enhance the effects of growth inhibition and they play an additive antitumor effect in hepatocellular carcinoma.