| 补肾活血方对成骨细胞增殖等生物学特性影响的实验研究 |
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| 引用本文： | 臧洪敏 陈君长 刘亦恒 刘润侠 王坤正. 补肾活血方对成骨细胞增殖等生物学特性影响的实验研究[J]. 中药材, 2005, 28(9): 803-805 |
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| 作者姓名： | 臧洪敏 陈君长 刘亦恒 刘润侠 王坤正 |
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| 作者单位： | [1]西安交通大学第二医陀骨科,西安710004 [2]西安交通大学第二医院中医科,西安710004 |
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| 摘 要： | 目的:观察中药补肾活血方对体外培养SD大鼠成骨细胞增殖、分化、矿化的的影响.方法:取新生的SD大鼠的颅骨,胶原酶法培养成骨细胞.应用MTT法,对硝基苯磷酸盐法(PNPP)及茜素红染色方法观察补肾活血方对体外培养成骨细胞的增殖、碱性磷酸酶(ALP)活性及矿化结节形成的影响.结果:补肾活血方具有刺激成骨细胞增殖,提高碱性磷酸酶活性及矿化结节形成的数量的作用(P＜0.01或P＜0.05).结论:补肾活血方具有促进成骨细胞的增殖、分化及矿化作用.
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| 关 键 词： | 补肾活血方 细胞培养 成骨细胞 |
| 收稿时间： | 2005-04-20 |
| 修稿时间： | 2005-04-20 |
Experimental Study of the Effect of Bushen Huoxue Recipe on Biological Characters of Osteoblasts in Vitro |
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| Zang Hongmin, Chen Junchang, Liu Yiheng,Liu Runxia, Wang Kunzheng. Experimental Study of the Effect of Bushen Huoxue Recipe on Biological Characters of Osteoblasts in Vitro[J]. Journal of Chinese medicinal materials, 2005, 28(9): 803-805 |
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| Authors: | Zang Hongmin Chen Junchang Liu Yiheng Liu Runxia Wang Kunzheng |
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| Affiliation: | Department of Orthopedics, Second Hospital of Xi' an Jiaotong University, Xi' an 710004. |
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| Abstract: | Objective:To observe the effect of Bushen Huoxue Recipe on cell proliferation, differentiation, maturation and minerali-zation in rats cultured osteoblasts. Methods: Osteoblasts from craniums of newly born SD rats were cultured in vitro. MTT, PNPP and ARS were used to observe the proliferation,activity of ALP and the number of mineral node of cultured osteoblasts in vitro. Results: It was found that Bushen Huoxue Recipe had the effect on stimulating cell proliferation, activity of ALP and the number of mineral node of cultured osteoblasts (P<0. 01 or P<0.05). Conclusion: Bushen Huoxue Recipe can promote proliferation, differentiation, maturation and mineralization of the osteoblasts cultured in vitro. |
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| Keywords: | Bushen Huoxue Recipe Osteoblasts Cell cultured |
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