CCK mRNA expression in neuroendocrine CRH neurons is increased in rats subjected to an immune challenge |
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Authors: | Juaneda C Lafon-Dubourg P Ciofi P Sarrieau A Wenger T Tramu G Corio M |
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Institution: | Laboratoire de Neurocytochimie Fonctionnelle, Université Bordeaux I, EA 2972, avenue des Facultés, 33405 cedex, Talence, France. |
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Abstract: | The expression of cholecystokinin (CCK) mRNA in neuroendocrine corticotropin-releasing hormone (CRH) neurons of the hypothalamic paraventricular nucleus (PVN) of male rats was examined 8 h following an acute immune challenge by intraperitoneal lipopolysaccharide (LPS, 250 microg/kg). Both quantitative, macroautoradiographic, single-label radioactive in situ hybridization histochemistry (ISHH) and qualitative dual-label ISHH were performed. Compared to controls, LPS-injected rats displayed increased (185%) parvicellular CCK mRNA expression levels, occurring in a majority (70%) of CRH neurons as revealed by dual-label ISHH. |
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