重组人生长激素对烧伤大鼠肠道细菌/内毒素移位的影响

目的观察早期应用重组人生长激素（recombinant human growth hormone， rhGH）对严重烧伤大鼠肠道细菌/内毒素移位的影响。方法30只成年雄性Wistar大鼠随机分为对照组、烧伤组和rhGH 3组。后2组造成25%总体表面积（TBSA）Ⅲ度烫伤，立即腹腔注射地塞米松80mg/kg，伤后2h分别给予等渗盐水和rhGH 1.33 IU/kg。伤后8、24h，观察肠道细菌移位频率、门静脉与腔静脉血清内毒素含量、腔静脉血清肿瘤坏死因子（TNF）α含量和肝功能变化。结果rhGH组肠道细菌移位频率和组织细菌含量均明显低于烧伤组（P<0.05），与对照组比较差异无统计学意义（P>0.05）；rhGH组门、腔静脉内毒素含量明显低于烧伤组（P<0.01）；腔静脉TNFα含量和肝功能指标明显低于烧伤组（P<0.01，P＜0.05）。结论早期应用大剂量rhGH能有效减轻严重烧伤后肠道细菌/内毒素移位，减少炎性介质释放，保护脏器功能。

Effect of recombinant human growth hormone on intestinal bacteria/endotoxin translocation in rats with burns

To investigate the effects of recombinant human growth hormone (rhGH) on intestinal bacteria/endotoxin translocation in early severely burned rats. Methods 30 adult male Wistar rats were randomly divided into three groups: the control group, the burn group and the rhGH treatment group. Rats in the burn and the rhGH treatment groups were inflicted with 25% total body surface area (TBSA) full-thickness skin scald on their backs immediately followed by intraperitoneal injection of dexamethasone (80mg/kg), then they were subcutaneously subjected to normal saline and rhGH (1.33 IU/kg) 2 hours after burning. Changes of the intestinal bacteria translocation rate, the endotoxin level in the portal and cava veins, the level of tumor necrosis factor (TNF) α in the cava venous serum and hepatic function were determined 8 and 24 hours after burning. ResultsThe intestinal bacteria translocation rate and the bacterial level in the rhGH group were significantly lower than those of the burn group (P<0.05), but were not significantly different from those of the control group (P>0.05). The endotoxin level in the rat portal and cava veins of the rhGH group was also significantly lower than that of the burn group (P<0.01). The level of TNFα in the cava vein and the indexes of hepatic function of the rhGH group were markedly lower than those of the burn group (P<0.01, 0.05). Conclusion A high dose of rhGH administered at an early stage following burning could effectively reduce intestinal bacteria/endotoxin translocation, decrease the release of inflammatory mediators and protect hepatic function.