腺病毒介导人血管抑素抑制乳腺癌生长的研究

目的：研究腺病毒介导的重组人血管抑制Plasminogen K5基因对乳腺癌的治疗作用。方法：通过PCR将人血纤维蛋白酶原的信号肽基因加至Plasminogen的K5结构域基因，克隆到质粒pCA13，并在293细胞中同源重组，得到腺病毒AdK5。将Ad-K5体外感染乳腺癌细胞B－Cap-37和血管内皮细胞ECV304，观察其对细胞生长的影响，同时在乳腺癌的裸鼠动物模型上，检测其对肿瘤生长的抑制作用。结果：在感染Ad-K5的B－Cap-37细胞中检测到K5基因mRNA的表达。Ad-K5对血管内皮细胞ECV304有抑制作用，但对癌细胞B-Cap-37没有直接抑制作用。动物实验表明Ad-K5能显著抑制肿瘤的生长。结论：K5基因能抑制乳腺癌实体瘤的生长。

Recombinant Kringle 5 of Human Plasminogen for Mammary Cancer Gene Therapy Mediated by Adenovirus

Objective: To investigate the inhibitory effects of Ad K5 on the proliferation of endothelial cells and the growth of mammary cancer. Methods: Kringle 5 of human plasminogen was constructed by PCR, and then cloned to plasmid pCA13. Recombinant adenovirus Ad K5 was obtained through homologous recombination in 293 cells. The inhibition effect of Ad K5 on cell proliferation was observed in vitro . The tumor size was measured at different intervals to observe the antitumoral effect of Ad K5 in vivo .Results: The mRNA expression of K5 gene was detected in Ad K5 infected B Cap 37 cells. Ad K5 inhibited the growth of ECV 304 cells, but had almost no effects on B Cap 37 cells. It seemed that Ad K5 could inhibit endothelial cells but not kill the cancer cells directly. Ad K5 could also inhibit the tumor growth in vivo. Conclusion: Ad K5 could inhibit the growth of solid mammary tumor.