补肾生血解毒方对免疫介导的再生障碍性贫血小鼠淋巴细胞分化及血液学参数的影响

目的探讨补肾生血解毒方对免疫介导的再生障碍性贫血(AA)小鼠淋巴细胞分化及血液学参数的影响。方法以~(60)Co-γ射线照射结合异源免疫细胞尾静脉注射致小鼠骨髓造血抑制模型,阳性对照药物免疫抑制剂环孢素A、中药补肾生血解毒方干预治疗,连续给药28 d。分别于第7、14、21、28天用血细胞计数仪检测外周血中血液学参数的变化;给药结束后用流式细胞仪检测小鼠外周血细胞中T细胞、B细胞、自然杀伤细胞(NK)比率的改变。结果与模型组相比,环孢素A组与补肾生血解毒方组在第21、28天,小鼠外周血中红细胞(RBC)、血红蛋白(HGB)、血小板(PLT)、白细胞(WBC)数量均升高(P0.05),差异有统计学意义。与模型组相比,补肾生血解毒方组大鼠外周血中CD3~+CD4~+细胞比率增多(P0.01),CD3~-CD19~+细胞和CD3~-NK1.1~+细胞比率降低(P0.01),差异有统计学意义。结论补肾生血解毒方能够通过调控AA小鼠淋巴细胞分化修复骨髓造血功能,达到治疗AA的目的。

Effects of Bushen Shengxue Jiedu Fang on lymphocyte differentiation and hematological parameters in mice with immune-mediated aplastic anemia

Objective To investigate the effects of Bushen Shengxue Jiedu Fang (Kidney-tonifying blood-generating toxin-removing Formula,BSSXJDF) on lymphocyte differentiation and hematological parameters in mice with immune-mediated aplastic anemia (AA).Methods Mice in all groups except the normal group received 60Co-γirradiation combined with injection of allogeneic immune cells in the tail vein to establish bone marrow hematopoietic inhibition model.The immunosuppressive cyclosporine A was used as the positive control drug,whereas Bushen Shengxue Jiedu Fang (BSSXJDF) was given to mice in the treatment groups for 28 consecutive days.Changes of hematological parameters after medication in the peripheral blood of mice were measured with cytometer at day 7,14,21 and 28.The ratio of T,B and NK cells was detected with flow cytometer.Results The count of red blood cells (RBC),hemoglobin (HGB),platelets (PLT) and white blood cells (WBC) in peripheral blood of mice were significantly increased (P ＜ 0.05) in BSSXJDF group compared with the model group;the ratio of CD3+ CD4+ cells in creased(P ＜ 0.01),and the ratio of CD3-CD19+ cells and CD3-NK1.1+ cells decreased (P ＜ 0.01) with statistical significance in the cyclosporine and BSSXJDF groups compored with the model group.Conclusion Bushen Shengxue Jiedu Fang can repair the hematopoietic function of bone marrow by regulating the differentiation of lymphocytes in the treatment of AA.