miR-137对肾癌GRC-1细胞增殖与凋亡的影响

目的 研究miR-137对肾癌GRC-1细胞增殖与凋亡的影响.方法 采用miR-137模拟物转染至肾癌GRC-1细胞,实验分为未转染组(未进行miR-137模拟物转染)、miR-137对照组(转染随机序列)、miR-137转染组(进行miR-137模拟物转染).荧光定量PCR检测各组转染后48h细胞中miR-137表达水平,应用噻唑蓝细胞增殖试验(MTT)、流式细胞检测技术与免疫荧光评价miR-137对肾癌GRC-1细胞增殖和凋亡的影响.结果 转染miR-137模拟物48h后,荧光定量PCR检测发现未转染组、miR-137对照组及miR-137转染组GRC-1细胞中miR-137的相对表达量依次为(24.43±2.03)％、(26.57±2.55)％、和(73.30±3.29)％,差异有统计学意义(P＜0.05).MTT细胞增殖实验与流式细胞仪检测结果显示,与未转染组、miR-137对照组相比,miR-137转染组转染48h后肾癌GRC-1细胞的增殖率显著降低,凋亡率显著升高,差异有统计学意义(P＜0.05),而未转染组、miR-137对照组肾癌GRC-1细胞的增殖率、凋亡率比较差异无统计学意义(P＞0.02).结论 miR-137可明显抑制肾癌GRC-1细胞增殖和促进其凋亡,故有望成为肾癌基因治疗的新靶点.

Effect of miR-137 on Proliferation and Apoptosis of Renal Carcinoma GRC-1 Cells

Objective To study the effect of miR-137 on proliferation and apoptosis of renal carcinoma GRC-1 cells.Methods miR-137 analogies were transfected into renal carcinoma GRC-1 cells.The non-transfection group(without transfection of miR-137 analogies),the miR-137 control group (transfected with random sequence) and the miR-137 transfection group(with transfection of miR-137 analogies) were used.The expression of miR-137 in cells was detected by fluorescence quantitative PCR in 48h after transfection.The effect of miR-137 on proliferation and apoptosis of renal carcinoma GRC-1 cells was evaluated with the methyl-thiazolyl-tetrazolium (MTT) test,flow cytometry and immunofluorescence.Results 48 hours after the transfection of miR-137 analogies,fluorescence quantitative PCR detected that the relative expression levels of miR-137 in GRC-1 cells in the non-transfection group,the miR-137 control group and the miR-137 transfection group were (24.43 ± 2.03) ％,(26.57 ± 2.55) ％ and (73.30 ± 3.29) ％,respectively (P ＜0.05).MTT cell proliferation assay and flow cytometry showed that the proliferation rate of renal carcinoma GRC-1 cells was significantly lower and the apoptosis rate was significantly higher in the miR-137 transfection group than the non-transfection group and miR-137 control group at 48 hours after transfection (P ＜0.05).However,there was no significant difference between the non-transfection group and miR-137 control group in the proliferation rate and apoptosis rate of renal carcinoma GRC-1 cells (P ＞ 0.02).Conclusion miR-137 can inhibit the proliferation and promote the apoptosis of GRC-1 cells,which might be a new target for gene therapy of renal carcinoma.