小檗碱体外抗子宫内膜癌HEC-1A细胞的作用研究

目的研究小檗碱体外对非激素依赖型人子宫内膜癌HEC-1A细胞增殖、迁移、周期、凋亡的影响。方法 MTT法检测小檗碱对人子宫内膜癌HEC-1A中分化腺癌,雌激素受体(ER)弱阳性,孕激素受体(PR)阴性]细胞株的抑制作用;倒置显微镜观察细胞形态变化;将实验组分成50、25、12.5 mg/L高、中、低剂量组。Transwell法测定细胞体外迁移能力,流式细胞仪观察细胞周期分布,Annexin V-FITC/PI双染检测HEC-1A细胞凋亡。结果小檗碱可显著抑制HEC-1A细胞生长,在100、50、25、12.5、6.25 mg/L浓度范围内呈剂量依赖性(P0.05)。小檗碱能明显改变细胞形态,小檗碱作用于子宫内膜癌HEC-1A细胞后,倒置显微镜下可见部分细胞体积改变,细胞内出现空泡,结构破坏,裂解成碎片,贴壁细胞减少,并散在排列。抑制子宫内膜癌HEC-1A细胞体外迁移的能力,高剂量组效果更佳,迁移细胞数明显减少。小檗碱低剂量组可以将HEC-1A细胞阻滞于G0/G1期。小檗碱作用24 h能提高HEC-1A细胞总凋亡率、早期凋亡率及晚期凋亡率,早期凋亡率更明显。结论小檗碱能够抑制非激素依赖型子宫内膜癌HEC-1A细胞株的增殖、迁移,并且促进HEC-1A细胞凋亡,低剂量小檗碱能将细胞阻滞在G0/G1期。

Effects of berberine on endometrial carcinoma HEC-1A cell line in vitro

Objective To study the effects of berberine on proliferation, migration, cell cycle and apop-tosis in hormone-independent endometrial carcinoma cell line HEC-1A. Methods Berberine' s inhibi-tion of HEC-1A cells proliferation was measured by using MTT colorimetry assay. Cell morphology was in-spected under an inverted microscope. Investigated Berberine groups were divided into high-dose group (50 mg/L), mid-dose group (25 mg/L), and low-dose group (12. 5 mg/L). Migration ability of cells in vitro was determined with Transwell. Cell cycle expressions were observed by using flow cytometry. Apoptosis of HEC-1A cells was detected with AnnexinV-FITC/PI double staining. Results Berberine may significantly inhibit the proliferation of HEC-1 A cells in a dose-dependent manner ( effective concen-tration of berberine ranged between 100 mg/L to 6. 25 mg/L) (P<0. 05). Berberine could markedly change cell morphology, which was visible under an inverted microscope, with vacuoles in cells, struc-tural damage even cracking into pieces, decreased adherent cells, and scattered alignment. Berberine could significantly inhibit HEC-1A cell migration especially in the high-dose group, on which amount the number of migrating cells decreased markedly. G0/G1 phase arrest was induced by low-dose group treat-ment after 24 hours. Berberine could accelerate total rate of apoptosis, early apoptosis rate and late apop-tosis rate of HEC-1A cells significantly; however, increase of early apoptosis rate was more apparrent. Conclusion Berberine could inhibit proliferation and migration of hormone-independent endometrial car-cinoma cell line HEC-1A, and improve the apoptosis rate of HEC-1A. Low-dose berberine may induce the cell at G0/G1 phase.