丹枝饮对盆腔炎性疾病后遗症小鼠JNK信号传导通路及相关炎性因子的影响

目的建立盆腔炎性疾病后遗症(SPID)小鼠模型,通过观察丹枝饮对IL-6、IL-8、单核细胞趋化蛋白-1(MCP-1)及巨噬细胞炎症蛋白-2(MIP-2)表达影响,以c-Jun氨基末端激酶(JNK)、磷酸化c-Jun氨基末端激酶(p-JNK)为观察指标,探讨丹枝饮药理作用及JNK信号传导通路的相关性。方法选用雌性小鼠24只,随机分为空白组、模型组、丹枝饮组及阿司匹林组。采用宫腔种植鼠衣原体方法建立SPID小鼠模型。给药治疗3个动情周期后小鼠眼眶取血,ELISA法检测IL-6及IL-8蛋白水平;Western Blot法检测子宫组织MCP-1、MIP-2、p-JNK、JNK相对蛋白量;免疫组化法检测子宫组织JNK磷酸化水平。结果与空白组比较,模型组IL-6、IL-8、MCP-1、MIP-2及JNK磷酸化水平升高(P0.01),差异有统计学意义;与模型组比较,丹枝饮组IL-6、IL-8、MCP-1、MIP-2及JNK磷酸化水平降低(P0.01),差异有统计学意义。结论丹枝饮的抗炎作用可能与调节SPID炎症机体血清中IL-6、IL-8含量相关,其抗炎作用机制可能是通过抑制JNK信号传导通路而下调MCP-1及MIP-2表达。

Effects of Danzhi Yin on mice with sequelae of pelvic inflammatory disease via JNK signal transduction pathway and inflammation-related cytokines

Objective To investigate the mechanism of Danzhi Yin (Pony Bark Jasmine Fruit Decoction,DZY) in treating mice with sequelae of pelvic inflammatory disease (SPID).Methods 24 mice were randomly divided into the blank group,model group,DZY group and aspirin group.The SPID model was established by intrauterine infection with chlamydia muridarum.After treatment,serum levels of IL-6 and IL-8 were detected by using ELISA.The protein expression of MCP-1,MIP-2,and p-JNK were detected with Western blot.The protein phosphorylation of JNK was measured with immunochemistry technology.Results Compared with blank group,IL-6,IL-8,p-JNK,MCP-1,and MIP-2 levels in the model group were significantly higher (P ＜ 0.01).Compared with model group,IL-6,IL-8,p-JNK,MCP-1,and MIP-2 levels in the DZY group were significantly lower (P ＜ 0.01).Conclusion DZY may play an anti-inflammatory role by regulating the levels of cytokines (IL-6,IL-8) in serum.DZY may inhibit the inflammation through suppression of JNK signal transduction pathway.