| HPLC同时测定六味补血颗粒中芍药苷和阿魏酸的含量 |
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| 引用本文： | 王文渊,龙红萍,卜生高.HPLC同时测定六味补血颗粒中芍药苷和阿魏酸的含量[J].湖南师范大学学报(医学版),2008,5(1). |
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| 作者姓名： | 王文渊 龙红萍 卜生高 |
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| 作者单位： | 1. 湖南永州职业技术学院药学系,湖南,永州,425006
2. 湖南永州市药品检验所,湖南,永州,425006 |
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| 摘 要： | 目的:建立同时测量六味补血颗粒中主药有效成分芍药苷和阿魏酸含量的方法。方法:采用Symmetry C18色谱柱(4.6×250 mm,5μm);流动相采用甲醇-0.2%磷酸溶液梯度洗脱;流速1.0 mL/min;检测波长为285 nm;柱温:25℃。结果:芍药苷在17.44~174.4μg.mL-1的范围内峰面积积分值与浓度呈良好的线性关系(r=0.9997),平均回收率为98.9%,RSD=1.29%(n=6);阿魏酸在0.356~14.24μg.mL-1的范围内峰面积积分值与浓度呈良好的线性关系(r=0.9999),平均回收率为98.68%,RSD=2.4%(n=6)。结论:该法准确、简便,重现性好,可作为六味补血颗粒制剂质量控制的有效方法。
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| 关 键 词： | 六味补血颗粒 芍药苷 阿魏酸 含量测定 |
HPLC Determination of Paeoniflorin and Ferulic Acid in Liuweibuxue Granules |
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| WAGN Wen-yuan,LONG Hong-ping,BU Shengg-ao.HPLC Determination of Paeoniflorin and Ferulic Acid in Liuweibuxue Granules[J].Journal of Hunan Normal University(Medical Science),2008,5(1). |
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| Authors: | WAGN Wen-yuan LONG Hong-ping BU Shengg-ao |
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| Institution: | WAGN Wen-yuan1,LONG Hong-ping1,BU Sheng-gao2 |
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| Abstract: | Objective to establish an HPLC method for determination of Paeoniflorin and ferulic acid in Liuweibuxue granules.Methods The analytical column was Symmetry C18(4.6×250 mm,5 μm) filled with at 5 μm stationary phase.Methanol-0.2% phosphoric acid gradient elution as mobile phase.The flow rate was 1.0 mL·min-1,the detection wavelength was 285 nm,and the column temperature was 25 ℃.Results The linear range for Paeoniflorin and ferulic acid were 17.44~174.4 μg·mL-1(r=0.9997) and 0.356~14.24 μg·mL-1(r=0.9999),the ... |
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| Keywords: | liuweibuxue granules ferulic acid paeoniflorin determination |
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