| bFGF及其受体FGFRS在成年大鼠唾液腺中的免疫组化定位和mRNA表达 |
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| 引用本文： | 黄永清,曲延征,马敏,何涛,陈伟辉. bFGF及其受体FGFRS在成年大鼠唾液腺中的免疫组化定位和mRNA表达[J]. 现代口腔医学杂志, 2004, 18(4): 296-298 |
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| 作者姓名： | 黄永清 曲延征 马敏 何涛 陈伟辉 |
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| 作者单位： | 1. 750004,银川,宁夏医学院附属医院口腔科
2. 福建医科大学附属协和医院 |
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| 摘 要： | 目的 本文研究碱性成纤维细胞生长因子[basic fibroblast growth factor(bFGF)]和成纤维细胞生长因子受体1、2、3和4(FGFR1、FGFR2、FGFR3和FGFR4)在大鼠唾液腺中分布及表达特点。方法 应用免疫组化和RT-PCR的方法研究bFGF和FGFR1，FGFR2，FGFR3和FGFR4在成年大鼠腮腺和颌下腺中的免疫组化定位和mRNA表达。结果 bFGF和FGFR1，FGFR2，FGFR3和FGFR4在成年大鼠腮腺和颌下腺的各级腺管上皮细胞均有较强的特异性免疫染色。在成年大鼠腮腺和颌下腺可以检测到bFGF和FGFR1，FGFR2，FGFR3mRNA表达。结论 成年大鼠腮腺和颌下腺是合成、分泌bFGF的重要源泉。
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| 关 键 词： | 成年大鼠 FGFR1 mRNA表达 腮腺 颌下腺 唾液腺 FGFR3 分泌 成纤维细胞生长因子受体 RT-PCR |
Immunohistochemical localization and mRNA expression of basic fibroblast growth factor(bFGF) and FGF receptors in rat salivary glands |
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| HUANG Yongqing,QU Yanzheng,MA Min,et al.. Immunohistochemical localization and mRNA expression of basic fibroblast growth factor(bFGF) and FGF receptors in rat salivary glands[J]. Journal of Modern Stomatology, 2004, 18(4): 296-298 |
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| Authors: | HUANG Yongqing QU Yanzheng MA Min et al. |
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| Affiliation: | HUANG Yongqing,QU Yanzheng,MA Min,et al. Department of Stomatology,Affiliated Hospital,Ninggxia Medical College,Yinchuan 750004 |
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| Abstract: | Objective To study the immunohistochemical localization and mRNA expression of basic fibroblast growth factor(bFGF) and FGFR1,FGFR2,FGFR3,FGFR4 in adult rat salivary glands.Methods Immunohistochemistry and RT-PCR methods were used to detect the immunoreactivity localization and mRNA expression of bFGF and FGFR1,FGFR2,FGFR3,FGFR4 in adult rat parotids and submandibular glands.Results Immunohistorchemical localization of bFGF and FGFRS in rat salivary glands revealed specific immunostaining of the ductal cells of the parotid and submandibular glands.RT-PCR amplification of total RNA from the parotid and submandibular glands of rats demonstrated the presence of bFGF and FGFR1,FGFR2,FGFR3 mRAN.Conclusion It was suggestde that bFGF and FGFRS could be endogenous synthesis by rat salivary glands.Thus,salivary glands appear to be an exocrine source of the bFGF in rat saliva. |
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| Keywords: | Salivary glands bFGF FGFRS |
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