全自动血细胞分析仪稀释模式用于脑脊液细胞计数及分类检测的可能性

目的：探讨ABX PENTRA 60血细胞分析仪在脑脊液细胞计数及分类中应用的可能性。方法：采用ABX PENTRA 60血细胞分析仪稀释模式对100份脑脊液标本进行白细胞计数、红细胞计数和白细胞分类分析，并与人工显微镜计数分类法结果进行对比。白细胞分类手工法采用瑞氏染色法，仪器法采用荧光染色和流式细胞术原理进行分类。结果：两种方法对脑脊液标本白细胞计数、红细胞计数、单个核及多个核细胞的绝对计数检测差异无统计学意义（ P>0.05），并且两种方法具有良好的相关性（白细胞计数r=0.988、红细胞计数r=0.988、单个核细胞绝对计数r=0.953，多个核细胞绝对计数r=0.981）。结论：应用ABX PENTRA 60血细胞分析仪的稀释模式进行脑脊液细胞计数、分类快速准确。全自动血细胞分析仪稀释模式检测脑脊液常规中细胞计数及分类可在临床应用中推广。

The Possibility of Automatic Blood Cell Analyzer Dilution Model for Cerebrospinal Fluid Cell Count and Classification Detection

Objective： To evaluate the of ABX PENTRA 60 automatic blood cell analyzer in white cell count and classification of cerebrospinal fluid CSF）. Methods： The diluted model of ABX PENTRA 60 automatic blood cell analyzer was used to detect the white cell count and classification in 100 samples of CSF, and compared to the results obtained with manually microscopic counting method. White blood cell classification was performed by manual method under the microscope , and instrumental method with fluorescence staining and flow cytometry. Results： There was no statistical difference between instrumental and manual method in the counting of WBC, RBC, mononuclear cell and multinucleate cells （P 〉0.05）. And the good correlation existed between the two methods （0. 988,0. 988,0. 953and 0. 981 for WBC, RBC ,mononuclear cell and multinucleate cell counts respectively）. Conclusions： The fluid model of ABX PENTRA 60 automatic blood cell analyzer is rapid and accurate in white cell count and classification of CSF. The diluted model of ABX PENTRA 60 automatic blood cell analyzer in WBC and classification of CSF has the value of popularization in clinical application.