| 应用抑制消减杂交技术研究鼠疫菌与假结核菌基因组之间的差异 |
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| 引用本文： | 王效义,秦珑,戴二黑,童宗中,韩延平,张建国,郭兆彪,宋亚军,周冬生,王津,王俊,杨瑞馥. 应用抑制消减杂交技术研究鼠疫菌与假结核菌基因组之间的差异[J]. 军事医学科学院院刊, 2005, 29(1): 18-20,24 |
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| 作者姓名： | 王效义 秦珑 戴二黑 童宗中 韩延平 张建国 郭兆彪 宋亚军 周冬生 王津 王俊 杨瑞馥 |
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| 作者单位： | 1. 军事医学科学院微生物流行病研究所,北京,100071
2. 中国科学院北京基因组研究所,北京,100101 |
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| 基金项目： | 国家“863”基金资助课题 |
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| 摘 要： | 目的：研究古典型鼠疫菌与假结核菌ATCC29833基因组间的差异，为进一步认识鼠疫菌的起源与进化奠定基础。方法：通过抑制消减杂交技术比较两种细菌基因组间的差异。结果：与已测序的3株鼠疫菌基因组进行同源性比较，发现了259个假结核菌基因组中存在的特异序列，与基因库进行同源性比较发现了10个假结核菌ATCC29833基因组中存在的新序列。结论：抑制消减杂交技术是一个简单而有效的比较近源微生物基因组之间差异的方法，鼠疫菌在进化过程中失去了大量的基因，假结核菌基因组之间也存在着一定的差别。
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| 关 键 词： | 鼠疫耶尔森氏菌 假结核耶尔森氏菌 基因组 抑制消减杂交技术 |
| 文章编号： | 1000-5501(2005)01-0018-04 |
Genomic comparison of Yersinia pestis and Yersinia pseudotuberculosis by suppression subtractive hybridization |
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| WANG Xiao-yi,QIN Long,DAI Er-Hei,TONG Zong-Zhong,HAN Yan-Ping,ZHANG Jian-Guo,GUO Zhao-biao,SONG Ya-Jun,ZHOU Dong-sheng,WANG Jin,WANG Jun,YANG Rui-fu. Genomic comparison of Yersinia pestis and Yersinia pseudotuberculosis by suppression subtractive hybridization[J]. Bulletin of the Academy of Military Medical Sciences, 2005, 29(1): 18-20,24 |
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| Authors: | WANG Xiao-yi QIN Long DAI Er-Hei TONG Zong-Zhong HAN Yan-Ping ZHANG Jian-Guo GUO Zhao-biao SONG Ya-Jun ZHOU Dong-sheng WANG Jin WANG Jun YANG Rui-fu |
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| Affiliation: | WANG Xiao-Yi1,QIN Long1,DAI Er-Hei1,TONG Zong-Zhong2,HAN Yan-Ping1,ZHANG Jian-Guo2,GUO Zhao-Biao1,SONG Ya-Jun1,ZHOU Dong-Sheng1,WANG Jin1,WANG Jun2,YANG Rui-Fu 1* |
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| Abstract: | Objective: To reveal the difference between the genome of Yersinia pestis and that of Y. pseudotuberculosis for providing insight into the origin and evolution of Y. pestis. Methods: Genomic comparison between Y. pestis and Y. pseudotuberculosis was conducted by suppression subtractive hybridization(SSH). Results: A total of 259 fragments specific to Y. pseudotuberculosis ATCC 29833 were found based on sequence homologous analysis against three whole-genome sequences of Y. pestis, and 10 new fragments unique to Y. pseudotuberculosis ATCC 29833 were found based on sequence homology analysis against GenBank database. Conclusions: SSH technique is a simple and efficient tool for identifying genetic differences between the closely related strains of bacteria. A number of genes are deleted from Y. pseudotuberculosis during its evolution to Y. pestis, and Y. pseudotuberculosis has undergone genomic flux to some extent. |
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| Keywords: | Yersinia pestis Yersinia pseudotuberculosis genome suppression subtractive hybridization (SSH) |
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