目测法酶联免疫吸附试验检测肺吸虫病特异抗体

<正> 酶联免疫吸附试验用于检测肺吸虫病血清特异抗体国内已有多篇报导。该法灵敏性高,操作简便,试剂用量少,适于临床检验及流行病学过筛。试验时将最终产物呈色反应液通过分光光度计测定其吸收值,以判断阳性及阴性的界限。但分光光度计一般实验室及基层目前尚难普及。因此,如能用肉眼观察代替分光光度测定,对该法的推广使用更有普及价值。于是我们试用目视法

USE OF VISUAL ASSAY IN ENZYME-LINKED IMMUNOSORBENT ASSAY(ELISA) FOR DETECTING SPEOIFIC ANTIBODIES OF PARAGONIMIASIS

Enzyme-linked immunosorbent assay (ELISA) done with soluble adult worm antigens of paragonimus is successful in detecting specific antibodies of paragonimiasis. The original technique had to be performed with spectrophotometer. In the present paper staphylococcal protein A was labelled with perox- idase. Both detecting procedures (visval assay and spectrophotometer assay) were employed at the same time for a population of 212 from paragonimiasis endemic areas and their positive rates were found to be 41.98％, 42.5％ (89/212, 96/211) respectively. It was found that visual assay was similar in sensitvity to spectrophotometer assay. Of the paragonimiasis patients, 96.8％ (60/62) showed a positive reaction. Serums from normal persons were simultaneously tested and false positive rate was 1.8％ (2/105). However, the visual assay may be used as serodiagnostic and seroepidemiologic tool for screening in epidemic of paragonimiasis. In addition, the preparation and store of positive and negative standardized sera were briefly discussed.