树突状细胞在体外诱导FBL-3细胞分化为单核细胞的研究

目的 探讨树突状细胞(DC)对白血病细胞的诱导分化作用机制.方法 应用不同浓度的DC培养上清作用于小鼠红白血病细胞(FBL-3细胞),作用72 h后用瑞氏染色观察分化后细胞的大体形态,透射电镜观察分化后细胞的超微结构,流式细胞术观察分化后细胞表面分子CD14的表达情况.结果 瑞氏染色、透射电镜、流式细胞术结果均显示,DC培养上清能诱导白血病细胞分化为单核细胞,其作用强度与DC培养上清中IL-12浓度呈正相关.结论 DC培养上清可诱导FBL-3细胞分化成为单核样细胞,分化后的细胞在大体形态、超微结构、细胞表型分子标志方面符合单核细胞的特点.且DC培养上清诱导FBL-3细胞分化成为单核样细胞的能力与培养上清中IL-12的浓度呈正相关.

Study of the mechanism of dendritic cells induce FBL-3 cells to differentiate into the monocytes in vitro

Objective To investigate mechanism involved in the differentiation of FBL-3 cells(eryth-roleukemia cells)induced by dendritic cells(DC). Methods To cultural the FBL-3 cells together with the DC supematant of different concentration for 72 hours,then we used the Wright's staining methed to record the ma-ture monocyte cell population, the transmission electron microscope to observe the ultramicrostructure, the flow eytoraetry to detect the expression rate of the surface molecular CD14. Results The Wright's staining methed, the transmission electron microscope and the flow cytometry all presented that after being induced by the super-natant of the DC ,the FBL-3 cells can partly differentiate into monocytes,inversion monocyte consistent with the personal characteristics. And the intensity of the DC supernatant was connected with interleukin-12. Conclusion The DC supernatant can induce the FBL-3 cells into monocytoid cell, the differentiated cells correspond to monocyte in macro-appearance,uhramicrostructure and phenotype. The competence of the DC supematant are partly concerned with interleukin-12.