抵抗素在大鼠急性胰腺炎中的表达

目的检测抵抗素在大鼠急性胰腺炎（AP）中的表达，探讨其与胰腺病理改变的关系。方法40只SD大鼠按数字表法随机分为正常组、假手术组、急性水肿性胰腺炎（AEP）组和急性坏死性胰腺炎（ANP）组。AEP组腹腔注射雨蛙素，ANP组腹腔注射精氨酸，正常组未做任何处理，假手术组腹腔注射等容量生理盐水。测定大鼠血清淀粉酶、抵抗素、TNF-α、IL-1β、C反应蛋白（CRP）水平，记录胰腺／体重比值，观察胰腺组织病理变化，免疫组化法检测胰腺组织抵抗素蛋白表达，实时定量PCR法检测胰腺组织抵抗素mRNA表达。结果抵抗素主要定位于胰腺腺泡细胞的胞质内。AEP组血清淀粉酶水平、胰腺／体重（g／kg）比值、胰腺组织病理评分、抵抗素mRNA表达量及血清抵抗素、IL-1β、TNF-α和CRP水平分别为（4377±343）U／L、（8．67±1．43）、（5．39±0．26）、（2．04±0．19）、（10．21±1．34）ng/ml、（184．18±45．24）pg／ml、（194．24±44．81）pg／ml、（3586±63）ng／ml；ANP组分别为（6750±322）U／L、（9．33±1．76）、（7．81±0．28）、（3．29±0．30）、（15．14±0．84）ng／ml、（349．31±94．54）pg／ml、（315．59±37．04）pg／ml、（4345±244）ng／ml。两组均显著高于假手术组的（1442±183）U／L、（4．34±0．42）、（1．10±0．21）、（0．88±O．08）、（5．13±0．74）ng/ml、（108．74±31．03）pg／ml、（106．44±21．31）pg／ml和（2895±165）ng／ml（P〈0．01），且该两组间差异也有统计学意义（P〈0．01或P〈0．05）。血清抵抗素水平与CRP、TNF-α、IL-1β水平及胰腺组织病理评分均呈正相关，相关系数r分别为0．711、0．871、0．794和0．812（P〈0．01）。结论抵抗素与AP的发生、发展有关，其检测结果可能是评估AP严重程度的有价值的指标之一.

Expression of resistin in rats of acute pancreatitis

Objective To investigate the expression of resistin in rats of acute panereatitis and the relationship between its expression and pancreatic pathologic changes. Methods Forty Sprague-Dawley rats were randomly divided into four groups： control group, sham operation group, AEP group and ANP group. AEP model was induced by intraperitoneal injection of cerulein and ANP model was induced by intraperitoneal injection of L-arginine while the control group received no treatment, and the sham operation group received same volume injections of 0.9% saline solution. The serum amylase, C-reactive protein, TNF-alpha, IL-1 beta were determined by using ELISA, pancreas/body weight （g/kg） ratio was recorded, pancreatic pathology was examinated, the expression of resistin mRNA and protein in pancreas was detected by real-time PCR and immunohistochemistry. Results The levels of amylase, pancreas/body weight ratio, pancreatic pathology score, expression of resistin mRNA, IL-1-beta, TNF-alpha and C-reactive protein in AEP group were （4377 ± 343）U/L, 8.67±1.43, 5.39 ±0.26, 2.04±0. 19, （10.21 ±1.34）ng/ml, （184. 18 ±45.24） pg/ml, （ 194.24 ± 44.81 ） pg/ml, （3586 ± 63） ng/ml, respectively; and the corresponding values in ANP group were（6750 ±322）U/L, 9.33 ±1.76, 7.81 ±0.28, 3.29 ±0.30, （15.14 ±0.84） ng/ml, （349.31 ±94.54）pg/ml, （315.59 ± 37.04） pg/ml, （4345 ± 244） ng/ml, respectively ; which were significantly higher than （ 1442 ± 183）U/L, 4.34±0.42, 1.10±0.21, 0.88±0.08, （5. 13 ± 0. 74 ） ng/ml , （108.74±31.03） pg/ml, （ 106.44 ± 21.31 ） pg/ml, （2895 ± 165 ） ng/ml in sham operation group （ P 〈 0.01 or P 〈 0.05 ）. Resistin was positively correlated with the increase of C-reactive protein, TNF-alpha, IL-1 beta and the pathologic score of pancreas, respectively （ r = 0. 711, 0. 812, 0. 794, 0. 812, P 〈 0.01 ）. Conclusions Resistin was correlated with the pathogenesis and development of acute pancreatitis, and may be useful to predict the severity of acute pancreatitis.