侧柏总黄酮的抗炎作用及机制

目的　研究侧柏总黄酮的抗炎作用及其作用机制。方法　以二甲苯致炎小鼠耳片肿胀及角叉菜胶诱发大鼠足爪肿胀模型分别探讨侧柏总黄酮对小鼠耳片肿胀及大鼠足爪肿胀的抑制作用。高效液相色谱法测定角叉菜胶致炎的大鼠中性粒细胞花生四烯酸代谢产物白三烯B4(LTB4)及 5 羟廿碳四烯酸 (5 HETE) ,荧光法测定 β 葡糖苷酸酶释放。结果　黄酮为 12 5,2 5 0 ,50 0mg·kg- 1,氢化可的松为3 5 0mg·kg- 1的剂量对二甲苯诱导的小鼠耳肿胀抑制率分别为 2 5 2 % ,3 5 1% ,47 7%和 66 5%。 2 5～ 50mg·kg- 1剂量能抑制大鼠足爪肿胀 ,2 5mg·kg- 1的总黄酮作用强于 2mg·kg- 1的地塞米松。浓度为 5 0～ 12 5 0mg·L- 1总黄酮对大白鼠中性粒细胞花生四烯酸代谢产物 5 HETE及LTB4生物合成的抑制率分别为 3 0 5%～ 87 3 %和 2 7 8%～84 3 %。 5 0 ,2 5 0 ,45 0mg·L- 1的总黄酮对A2 3 187诱导的 β 葡糖苷酸酶释放抑制率分别为 13 4% ,2 6 2 %和3 2 2 %。结论　侧柏总黄酮具有较强的抗炎作用 ,其对中性粒细胞花生四烯酸代谢产物LTB4 、5 HETE生物合成及 β 葡糖苷酸酶释放的抑制作用可能与其抗炎作用有关。

The anti-inflammatory effect of total flavonoids isolated from Platychadus orientalis and its mechanism

AIM To investigate the anti-inflammatory eff ec t of total flavonoids isolated from Platychadus orientalis and its mechanism . METHODS The inflammatory models in mice and swelling of rats hind paws were induced by dimethylbenzene and carrageenan respectivel y. The biosynthesis of leukotriene and β-glucuronidase release in the cells we re measured by HPLC and fluorescence spectrophotometer respectively. RES ULTS Total flavonoids isolated from Platychadus orientalis remarkably inhibited the ear edema induced by dimethylbenzene in mice at the dose of 12.5 ～50.0 mg·kg -1 and the swelling of rats hind paws induced by carrageenan at the dose of 25.0～50.0 mg·kg -1 . Total flavonoids at final concentrati on of 5.0～125.0 mg·L -1 inhibited 5-HETE and LTB 4 synthesis by 30.5%～ 87.3% and 27.8%～84.3% respectively. In comparision with controls, Total flavono ids at final concentration of 5.0～45.0 mg·L -1 inhibited β-glucuronidas e release by 13.4%～32.2%. CONCLUSION Total flavonoids isolated f rom Platychadus orientalis has an anti-inflammatory effect which may be rel ated to inhibiting 5-HETE and LTB 4 production and β-glucuronidase release.