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| 破伤风类毒素聚乳酸微球中蛋白质稳定性的研究 | |
| 引用本文： | 何应,柳林,魏树礼,王东升. 破伤风类毒素聚乳酸微球中蛋白质稳定性的研究[J]. 中国药学杂志, 2001, 36(6): 391-394 |
| 作者姓名： | 何应 柳林 魏树礼 王东升 |
| 作者单位： | 1. 北京大学药学院药剂教研室, 2. 北京大学医药卫生分析中心药学与化学分析室, 3. 北京大学基础医学院免疫教研室, |
| 基金项目： | 国家自然科学基金项目(39770878) |
| 摘 要： | 目的研究破伤风类毒素聚乳酸微球中破伤风类毒素在制备过程和体内酸性环境中的稳定性及微球中附加剂的稳定作用。方法采用分子排阻色谱法，十二烷基硫酸钠聚丙烯酰胺凝胶电泳法，双向免疫扩散法。结果在制备过程和体内酸性环境中破伤风类毒素活性有轻微下降；附加剂增加微球包封率，改善药物释放，稳定蛋白质活性，尤其是具有多羟基结构的附加剂。结论破伤风类毒素聚乳酸微球中的蛋白质在制备过程和体内酸性环境中基本稳定，聚乳酸微球可望发展成为疫苗控释系统。 |
| 关 键 词： | 破伤风类毒素 聚乳酸 微球 稳定性 附加剂 |
| 文章编号： | 1001-2494(2001)06-0391-04 |
| 收稿时间： | 2000-06-22; |
| 修稿时间： | 2000-06-22 |
Studies on the stability of protein in tetanus toxoid encapsulated in polylactidemicrospheres |
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| HE Ying ,LIU Lin ,WEI Shu li ,WANG Dong sheng. Studies on the stability of protein in tetanus toxoid encapsulated in polylactidemicrospheres[J]. Chinese Pharmaceutical Journal, 2001, 36(6): 391-394 | |
| Authors: | HE Ying LIU Lin WEI Shu li WANG Dong sheng |
| Affiliation: | HE Ying 1,LIU Lin 2,WEI Shu li 1,WANG Dong sheng 3 |
| Abstract: | OBJECTIVE To investigate the stability of microencapsulated tetanus toxoid in the courses of preparation and release, and improve its stability by co encapsulation of additives.METHODS High pressure size exclusion chromatography, sodium dodecyl sulfate polyacrylamide gel electrophoresis, double immunodiffusion were used.RESULTS The protein denaturation processes were ascribed to the course of preparation and release. Additives,especially the additives with polyhydroxy structures,enhancd the encapsulation efficiency in the microspheres,improved the release profiles and stabilized the active proteins,respectively.CONCLUSION The stability of tetanus toxoid loaded microspheres was satisfactory.The optimized tetanus toxoid containing microspheres may be valuable for a controlled vaccine delivery system. |
| Keywords: | tetanus toxoid polylactide microspheres stability additives |
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