高效液相色谱法测定法莫替丁的血药浓度

目的 :建立HPLC法测定法莫替丁的血药浓度。方法 :采用Nova_PakC18(3 9×150mm ,5μm )色谱柱 ,柱温35℃ ;乙腈 -醋酸铵缓冲液 (6∶94)为流动相 ;流速1 1ml/min ;检测波长266nm。血浆样品经液 -液萃取处理。结果 :法莫替丁的血药浓度在12 5～300ng/ml范围内 ,与其峰面积有良好的线性关系 (γ=0 9996)。最低检测浓度8ng/ml ,方法的平均回收率102 39 % ,日内精密度≤5 00 % ,日间精密度≤6 59 %。结论 :该方法灵敏、准确、经济 ,可用于法莫替丁的药代动力学及生物利用度研究。

Determination of Plasma Level of Famotidine by HPLC

OBJECTIVE:A method was established for the determination of Famotidine in plasma by HPLC METHODS:Analytical column was Nova-Pak C18(3 9×150mm,5μm),the mobile phase consisted of acetonitrile-ammoniun acetate buffer(6∶94),the flow rate was 1 1ml/min and column temperature was 35℃ The detection was performed at UV266nm Plasma samples were extracted with ethylacetate RESULTS:The calibration curve was linear in the concentrations ranged from 12 5 to 300ng/ml(γ=0 9 996) The detection limit was 8ng/ml The recovery was 102 4% The RSD within day was less than 5 0% and the RSD between days was less than 6 6% CONCLUSION:The method is accurate and can be used for studying the pharmacokinetics and bioavailability of Famotidine