| 原代白血病骨髓基质细胞对Jurkat细胞CRIF1基因表达的影响 |
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| 引用本文： | 李忠俊,陈幸华.原代白血病骨髓基质细胞对Jurkat细胞CRIF1基因表达的影响[J].中国输血杂志,2008,21(1):25-27. |
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| 作者姓名： | 李忠俊 陈幸华 |
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| 作者单位： | 第三军医大学,附属新桥医院输血科,重庆,400037 |
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| 基金项目： | 重庆市卫生局医学科研项目(NO:07-2-247)，第三军医大学校管课题资助(NO:2004D110) |
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| 摘 要： | 目的研究与原代白血病骨髓基质细胞共培养后。白血病细胞的CRIF1基因表达情况。方法:1)采用Percoll体外分离正常、初治白血病患者骨髓单个核细胞,贴壁培养骨髓基质细胞;2)应用RT-PCR技术检测白血病骨髓基质细胞屏蔽的Jurkat细胞CRIF1 mRNA表达的改变。结果发现Jurkat细胞CRIF1 mRNA有一定量的基础表达,白血病骨髓基质细胞屏蔽的Jurkat细胞CRIF1 mRNA的表达与对照组相比明显上调。结论CRIF1高表达可能与白血病骨髓基质细胞抑制白血病细胞增殖有关。
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| 关 键 词： | 骨髓基质细胞 细胞周期 Jurket细胞 CRIF1基因 |
| 文章编号： | 1004-549X（2008）01-0025-03 |
| 收稿时间： | 2007-07-24 |
| 修稿时间： | 2008-01-07 |
Expression level of CRIF1 in human leukemic Jurkat T-cell line cocultured with primary leukemic bone marrow stromal cells |
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| Li Zhongjun,Chen Xinghua.Expression level of CRIF1 in human leukemic Jurkat T-cell line cocultured with primary leukemic bone marrow stromal cells[J].Chinese Journal of Blood Transfusion,2008,21(1):25-27. |
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| Authors: | Li Zhongjun Chen Xinghua |
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| Abstract: | Objective To investigate the expression level of CRIF1 in human leukemic Jurkat T-cell line cocultured with primary leukemic bone marrow stromal cells.Methods RT-PCR was used to study the expression level of CRIF1 in human leukemic Jurkat T-cell line cocultured with primary leukemic bone marrow stromal cells in vitro. Results After coculture with leukemic bone marrow stromal cells, the expressions of CRIF1 and Gadd45 mRNA in Jurkat cells were significantly higher than the control.Conclusion High mRNA levels of CRIF1 might be associated with the G0/G1 phage arrest in human leukemic Jurkat T-cell line cocultured with primary leukemic bone marrow stromal cells. |
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| Keywords: | Bone marrow stromal cells Cell cycle CR6-interacting factor 1 |
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