Effects of Iptakalim on intracellular free calcium concentration of cultured rabbit pulmonary arterial smooth muscle cells |
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| Authors: | Xu Qi Weiping Xie Gang Hu Hai Wang Hong Wang |
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| Institution: | Xu Qi(Department of Respiratory Medicine, The First Affliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China)；Weiping Xie(Department of Respiratory Medicine, The First Affliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China)；Gang Hu(Department of Pharmacology and Neurobiology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China)；Hai Wang(Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China)；Hong Wang(Department of Respiratory Medicine, The First Affliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China)； |
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| Abstract: | Objective:To explore the effects of Iptakalim on intracellular free calcium concentration and on the proliferation of cultured rabbit pulmonary arterial smooth muscle cells induced by endothelin-1(ET-1) in vitro. Methods:A cell culture model, 3H]-thymidine(3H]-TdR) incorporation test and confocal microscope were used to observe proliferation and intracellular free calcium concentration(Ca2+]i) of rabbit PASMC induced by ET-1 in vitro. Results:The value of 3H]-TdR incorporation in ET-1 group was increased 1.468 times higher than that in control group. Iptakalim at the concentration of 10-7mol/L,10-6 mol/L,10-5 mol/L lowered 3H]-TdR incorporation by (19.8±4.6)%, (41.2±9.5)%, (54.7±10.1)%, respectively, compared with the value of the cells treated with ET-1(P＜0.01); The intracellular fluorescence intensity of PASMC in ET-1 group was increased from 73.70±10.12 to 143.84±28.23, significantly higher than that in control group(P＜0.01); whereas with Iptakalim,the fluorescence intensity(FI) was only increased from 74.30±10.20 to 86.03±9.82, significantly lower than that in ET-1 group(P＜0.01). Conclusion:Iptakalim inhibited proliferation of PASMC and decreased intracellular free calcium concentration of cultured rabbit PASMC induced by ET-1. |
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